QIAN Shanshan, FENG Xue, YU Tong, GUAN Mengshu, LI Jia, LIU Yue, XU Cong, HOU Juncai. Study on the Lipid-lowering Effect of Soybean Active Peptide on Obese Mice[J]. Science and Technology of Food Industry, 2021, 42(3): 310-314,319. DOI: 10.13386/j.issn1002-0306.2019100084
Citation: QIAN Shanshan, FENG Xue, YU Tong, GUAN Mengshu, LI Jia, LIU Yue, XU Cong, HOU Juncai. Study on the Lipid-lowering Effect of Soybean Active Peptide on Obese Mice[J]. Science and Technology of Food Industry, 2021, 42(3): 310-314,319. DOI: 10.13386/j.issn1002-0306.2019100084

Study on the Lipid-lowering Effect of Soybean Active Peptide on Obese Mice

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  • Received Date: October 15, 2019
  • Available Online: February 02, 2021
  • In this study,the ICR mice were divided into normal control group,high-fat model group and soybean peptide dose group. The dose of gavage was 200,400 mg/kg and 800 mg/kg respectively. The effects of soybean active peptide on obesity and dyslipidemia induced by high-fat were analyzed by analyzing the changes of body weight and blood lipid. The results showed that the body weight,Lee’s index,food intake,liver and adipose tissue weight of obese mice in the high-fat model group decreased significantly(P<0.05)after high dose of soybean active peptide was administered to the stomach. The contents of total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C)were significantly decreased(P<0.05),while the contents of high-density lipoprotein cholesterol(HDL-C)were significantly increased(P<0.05). The content of TC in liver decreased significantly(P<0.05),while that in feces increased significantly(P<0.05). Compared with the normal control group,there was no significant difference in body weight,Lee’s index,food intake,liver weight,fat coefficient,TC,TG,LDL-C and HDL-C in the high dose group(P>0.05),while the TC content in feces increased significantly(P<0.05). The results of liver tissue section showed that soybean active peptide could reduce the degree of fatty degeneration of liver,and the effect of high dose group was the most obvious. Therefore,the soybean polypeptide has a good weight-loss effect.
  • [1]
    刘静波,刘畅,赵颂宁,等. 不同分子质量的大豆肽螯合钙工艺优化[J]. 中国食品学报,2018,18(6):146-152.
    [2]
    乔惠敏. 大豆肽在动物生产中的作用及应用[J]. 四川畜牧兽医,2018,45(8):40-41.
    [3]
    Chen Z,Li W,Santhanam R K,et al. Bioactive peptide with antioxidant and anticancer activities from black soybean(Glycine max(L.)Merr.)byproduct:Isolation,identification and molecular docking study[J]. European Food Research and Technology,2019,245(3):677-689.
    [4]
    王立博,陈复生.大豆活性肽生理保健功能研究进展[J]. 食品与机械,2016,32(2):198-201.
    [5]
    Chen J R,Suetsuna K,Yamauchi F. Isolation and characterization of immunostimulative peptides from soybean[J]. Journal of Nutritional Biochemistry,1995,6(6):310-313.
    [6]
    Yamauchi F,Suetsuna K. Immunological effects of dietary peptide derived from soybean protein[J]. Journal of Nutritional Biochemistry,1993,4(8):450-457.
    [7]
    Tsuruki T,Kishi K,Takahashi M,et al. Soymetide,an immunostimulating peptide derived from soybean bconglycinin,is an fMLP agonist[J]. FEBS Letters,2003,540(1-3):206-210.
    [8]
    Xu L,Li H M,Huang Y B,et al. A study on anti-oxidative activity of soybean peptides,with linoleic acid peroxidation systems[J]. 高等学校化学研究(英文版),2006,22(2):205-208.
    [9]
    Tovar A R,Murguía,Fernanda,Cruz C,et al. A soy protein diet alters hepatic lipid metabolism gene expression and reduces serum lipids and renal fibrogenic cytokines in rats with chronic nephrotic syndrome[J]. Journal of Nutrition,2002,132(9):2562-2569.
    [10]
    Sirtori C R,Lovati M R,Manzoni C,et al. Soy and cholesterol reduction:Clinical experience[J]. Journal of Nutrition,1995,125(3):598S-605S.
    [11]
    Tovarpalacio C,Potter S M,Hafermann J C,et al. Intake of soy protein and soy protein extracts influences lipid metabolism and hepatic gene expression in gerbils[J]. Journal of Nutrition,1998,128(5):839-842.
    [12]
    王升光,于帅,孟凡刚,等. 酶法制备大豆肽的相对分子量分布及降压作用研究[J]. 食品工业科技,2018,39(1):46-51.
    [13]
    崔洪斌. 大豆生物活性物质的开发与应用[J]. 中国食物与营养,2000(1):15-17.
    [14]
    褚斌杰. 大豆肽的分离及其生理功能鉴定[D].武汉:华中农业大学,2011.
    [15]
    Zhong F,Zhang X,Ma J,et al. Fractionation and identification of a novel hypocholesterolemia peptide derived from soy protein Alcalase hydrolysates[J]. Food Research International,2007,40(6):756-762.
    [16]
    TakenakaY,Utsumi Sand,Yoshikawa M. Introduction of a low molecular weight agonist peptide for complement C3a receptor into soybean proglycinin A1aB1b subunit by site-directed mutagenesis[J]. Biosci Biotechnol Biochem,2001,65(5):1202-1205.
    [17]
    包乐媛,张业尼,钱磊,等. 大豆肽对高脂血症大鼠的降脂作用[J].大豆科学,2007,26(5):752-756.
    [18]
    陈栋梁,刘莉,黄刚,等. 紫苏油及大豆肽合剂对大鼠血脂的调节作用[J]. 临床心血管病杂志,2003,19(1):30-32.
    [19]
    杨晓,王畋,刘畅,等.大豆肽胶囊的安全性毒理学评价[J].食品研究与开发,2018,39(4):193-199.
    [20]
    李峰,潘瑶,陈奇.大豆活性肽酶解制备的工艺条件研究[J].食品科学,2010,31(10):69-74.
    [21]
    卢申姣,黄尔梦,黄洁虹,等.神秘果对高脂模型小鼠的降血脂效应[J].中药药理与临床,2018,34(2):75-79.
    [22]
    Folch J,Lees M,Stanley G H S. A simple method for the isolation and purification of total lipides from animal tissues[J]. Journal of Biological Chemistry,1957,226(1):497-509.
    [23]
    甄红敏. 燕麦灭酶对大鼠血糖、血脂和肠道的影响[D]. 杨凌:西北农林科技大学,2011.
    [24]
    Aoyama T,Fukui K,Takamatsu K,et al. Soy protein isolate and its hydrolysate reduce body fat of dietary obese rats and genetically obese mice(yellow KK)[J]. Nutrition,2000,16(5):349-354.
    [25]
    褚斌杰,祁高富,梁运祥.大豆肽减肥降血脂作用的研究[J].食品科技,2011,36(11):65-68.
    [26]
    刘恩岐. 黑豆蛋白酶解产物的生物活性研究与结构表征[D].杨凌:西北农林科技大学,2013.
    [27]
    孙青. 大豆肽对大鼠血脂代谢的影响及其抗动脉粥样硬化机理研究[D].济南:山东大学,2013.
    [28]
    黄谨. 鬼箭羽水提取物对非酒精性脂肪肝大鼠肝纤维化的影响[D].恩施:湖北民族学院,2018.
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