Separation and purification of total flavonoids from lane late navel orange by AB-8 macroporous resins

NIE Chao; ZHAO Zi-yan; XU Xiao-dan; ZHOU Zhi-qin

doi:10.13386/j.issn1002-0306.2017.18.042

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Science and Technology of Food Industry > 2017 > (18): 221-225. > DOI: 10.13386/j.issn1002-0306.2017.18.042

NIE Chao, ZHAO Zi-yan, XU Xiao-dan, ZHOU Zhi-qin. Separation and purification of total flavonoids from lane late navel orange by AB-8 macroporous resins[J]. Science and Technology of Food Industry, 2017, (18): 221-225. DOI: 10.13386/j.issn1002-0306.2017.18.042

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Separation and purification of total flavonoids from lane late navel orange by AB-8 macroporous resins

College of Horticulture and Landscape Architecture, Southwest University
Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education

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Received Date: February 27, 2017

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Abstract

The adsorption and desorption of total flavonoids from lane late navel orange fruits were investigated by using 3macroporous resins.Results indicated that AB-8 resin was appropriate to purify the total flavonoids.Then the polyphenols from orange fruits were qualitatively and quantitatively by HPLC ( high performance liquid chromatography) . To optimize the separation process, the static and dynamic of adsorption and desorption experiments were carried out, the optimal adsorption conditions were as follows: initial concentrations in sample solution 0.75 mg/m L, p H3.0, sample loading amount 3.5 BV, flow rate 2 BV/h.The optimal desorption parameters were 80% ethanol 5 BV, flow rate of 2 BV/h, the desorption ratio was 90.73%.After purification, the content of flavonoids in product was increased from 36.04 mg/g to 334.54 mg/g. HPLC analysis results found that the flavonoids profiles of lane late navel orange were not changed after adsorption and desorption by AB-8macroporous resin. The main flavonoids were narirutin and hesperidin, the percentage content to the total flavonoids was91.14%. The results showed that AB-8 resin revealed a good ability to enrichment total flavonoids from lane late navel orange, and the method can be referenced for the enrichment of total flavonoids from other material.
- lane late navel orange,
- total flavonoids,
- high performance liquid chromatography,
- macroporous resin,
- separation and purification

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[1]	谢宗周, 邓秀新, 伊华林, 等.晚熟脐橙新品种——伦晚脐橙的选育[J].果树学报, 2011 (4) :733-734, 548.
[2]	黄林华, 吴厚玖.我国水果副产物综合利用的研究及应用[J].食品安全质量检测学报, 2015 (11) :4446-4452.
[3]	丁晓波, 张华, 周志钦, 等.柑橘果品营养学研究现状[J].园艺学报, 2012 (9) :1687-1702.
[4]	Huang Y S, Ho S C.Polymethoxy flavones are responsible for the anti-inflammatory activity of citrus fruit peel[J].Food Chemistry, 2010, 119 (3) :868-873.
[5]	陈嘉景, 彭昭欣, 石梅艳, 等.柑橘中类黄酮的组成与代谢研究进展[J].园艺学报, 2016 (2) :384-400.
[6]	Zhou D Y, Xing R, Xu Q, et al.Polymethoxylated flavones metabolites in rat plasma after the consumption of Fructusaurantii extract:Analysis by liquid chromatography/electrospray ion trap mass spectrometry[J].Journal of Pharmaceutical and Biomedical Analysis, 2008, 46 (3) :543-549.
[7]	Dong Y, Zhao M, Sun-Waterhouse D, et al.Absorption and desorption behaviour of the flavonoids from Glycyrrhiza glabra L.leaf on macroporous adsorption resins[J].Food Chemistry, 2015, 168:538-545.
[8]	张旭, 王锦玉, 仝燕, 等.大孔树脂技术在中药提取纯化中的应用及展望[J].中国实验方剂学杂志, 2012 (6) :286-290.
[9]	汪洋, 吴剑, 曾凡坤, 等.大孔树脂纯化柑橘皮渣类黄酮的工艺研究[J].食品工业, 2011 (10) :34-37.
[10]	程灏旻, 杨祖全, 唐振洋, 等.大孔树脂对血橙皮中黄酮类化合物的纯化研究[J].广州化工, 2015 (4) :62-63, 89.
[11]	张元梅, 周志钦, 叶兴乾, 等.高效液相色谱法同时测定柑橘果实中18种类黄酮的含量[J].中国农业科学, 2012 (17) :3558-3565.
[12]	豆浩然, 倪健, 夏振文, 等.大孔树脂纯化展毛地椒总黄酮工艺研究[J].辽宁中医杂志, 2015 (9) :1726-1729.
[13]	骆党委, 叶静, 肖美添, 等.AB-8大孔吸附树脂精制芦柑皮总黄酮及黄酮类化合物的分离[J].食品科学, 2014 (6) :30-35.
[14]	廖春燕, 杨欣绿.D101型大孔树脂纯化鸡骨草总黄酮的工艺优选[J].中国实验方剂学杂志, 2013 (2) :38-40.
[15]	苗修港, 余翔, 梁峻彬, 等.NKA-9大孔树脂纯化香椿叶黄酮类物质工艺优化[J].食品科学, 2016 (8) :32-38.
[16]	Bi Y G, Tan Y Q.Study on Macroporous Resin Separation and Purification of Total Flavonoids of Plantago Process[C]//Advanced Materials Research.Trans Tech Publications, 2012, 550:987-992.
[17]	Zhao Z, Dong L, Wu Y, et al.Preliminary separation and purification of rutin and quercetin from Euonymus alatus (Thunb.) Siebold extracts by macroporous resins[J].Food and Bioproducts Processing, 2011, 89 (4) :266-272.
[18]	Zhang Y, Jiao J, Liu C, et al.Isolation and purification of four flavone C-glycosides from antioxidant of bamboo leaves by macroporous resin column chromatography and preparative highperformance liquid chromatography[J].Food Chemistry, 2008, 107 (3) :1326-1336.
[19]	童鑫, 张瑞芬, 张名位, 等.米糠酚类物质的大孔树脂分离纯化工艺[J].中国农业科学, 2016 (19) :3818-3830.
[20]	唐巧玉, 周毅峰, 阎婷.HPD300大孔树脂纯化金橘皮黄酮类化合物的工艺研究[J].食品科学, 2008 (8) :355-358.

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