Screening and identification of a marine bacteria producing chitin deacetylase and optimization of its fermentation conditions

GU Zhang-hui; LIU Shu; HU Sheng-yuan; LAI Jiang-li; WANG Shu-jun; JIAO Yu-liang; FANG Yao-wei

doi:10.13386/j.issn1002-0306.2017.18.025

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Science and Technology of Food Industry > 2017 > (18): 129-134. > DOI: 10.13386/j.issn1002-0306.2017.18.025

GU Zhang-hui, LIU Shu, HU Sheng-yuan, LAI Jiang-li, WANG Shu-jun, JIAO Yu-liang, FANG Yao-wei. Screening and identification of a marine bacteria producing chitin deacetylase and optimization of its fermentation conditions[J]. Science and Technology of Food Industry, 2017, (18): 129-134. DOI: 10.13386/j.issn1002-0306.2017.18.025

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Screening and identification of a marine bacteria producing chitin deacetylase and optimization of its fermentation conditions

College of Marine Science and Technology, Huaihai Institute of Technology
Jiangsu Marine Resources Development Research Institute

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Received Date: March 19, 2017

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Objective: Screen chitin-deacetylase-producing strains from marine samples, identify the strain and optimize its fermentation conditions.Methods: Chitin-deacetylase-producing strains were screened by color culture medium from the marine mud of Yanwei Harbor. The strains were identified by morphological, physiological and biochemical characteristics and16 S r DNA sequence analysis. The single factor and orthogonal experimental design were used to optimize the fermentation conditions.Results: Strain MCDA01 producing chitin deacetylase was screened, and it was identified as Acinetobacter schindleri through morphological, biochemical characteristics, and 16 S r DNA sequence analysis. The optimal culture conditions of strain MCDA01 was obtained as follows: 30 ℃, p H8.0, and Na Cl 4%.The optimal fermentation conditions was obtained as follows: the fermentation time was 60 h, the fermentation temperature was 20 ℃, the culture medium starts p H was 7, the liquid loading amount was 20%, and the inducer chitin concentration was 1%. Under this condition, production of enzyme was up to201.37 U/m L, which was increased by 4.14 times. Conclusion: The maximum of enzyme production were optimized to201.37 U/m L, and have industrial application potential.
- chitin deacetylase,
- strain screening,
- identifiction,
- fermentation optimization

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