Analysis of antigenic epitopes and cloning of soybean major allergen β-conglycinin beta-subunit

PI Jiang-yi; XI Jun; HE Meng-xue; LI Shuang

doi:10.13386/j.issn1002-0306.2017.18.018

Issue 18

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2017 > (18): 90-93. > DOI: 10.13386/j.issn1002-0306.2017.18.018

PI Jiang-yi, XI Jun, HE Meng-xue, LI Shuang. Analysis of antigenic epitopes and cloning of soybean major allergen β-conglycinin beta-subunit[J]. Science and Technology of Food Industry, 2017, (18): 90-93. DOI: 10.13386/j.issn1002-0306.2017.18.018

Citation:

PDF (1078 KB)

Analysis of antigenic epitopes and cloning of soybean major allergen β-conglycinin beta-subunit

College of Food Science and Technology, Henan University of Technology

More Information

Received Date: February 22, 2017

Graphical Abstract

Abstract

Abstract

The model of tertiary of β-conglycinin beta-subunit was constructed by the bioinformation tool SWISS-MODEL, and the conformational epitopes were predicted by Disco Tope 2.0 server.The overlapping fragments of β-conglycinin beta-subunit genes were amplified by PCR and inserted into p MD-18 T vector, then preserved into competent cell of Escherichia coli JM109.The recombinant plasmids were evaluated by blue-white selection, PCR and double enzyme digestion, and DNA sequence analysis showed that the cloned sequence was the very fragment designed. The successful cloning of the genes can provide a reference for the study of the expresstion of β-conglycinin beta-subunit and screening of antigenic epitopes.
- β-conglycinin,
- beta-subunit,
- bioinformation,
- antigenic epitopes,
- clone

FullText(HTML)

References (16)

References

[1]	ALOK V, SANDEEP K, MUKUL D, et al.A comprehensive review of legume allergy[J].Clinical Reviews in Allergy and Immunology, 2013, 45 (1) :30-46.
[2]	HE L, HAN M, QIAO S, et al.Soybean Antigen Proteins and their Intestinal Sensitization Activities[J].Current Protein and Peptide Science, 2015, 16 (7) :613-621.
[3]	SUN X, SHAN X, YAN Z, et al.Prediction andcharacterization of the linear Ig E epitopes for the major soybeanallergenβ-conglycinin using immunoinformatics tools[J].Food and Chemical Toxicology, 2013, 56 (12) :254-260.
[4]	Maruyama N, Adachi M, Takahashi K, et al.Crystal structures of recombinant and native soybean beta-conglycinin beta homotrimers[J].European Journal of Biochemistry, 2001, 268 (12) :3595-3604.
[5]	MEINLSCHMIDT P, SCHWEIGGERT-WEISZU, BRODE V, et al.Enzyme assisted degradation of potential soy protein allergens with special emphasis on the technofunctionality and the avoidance of a bitter taste formation[J].LWT-Food Science and Technology, 2016, 68:707-716.
[6]	朱婷伟, 陈复生, 布冠好, 等.大豆蛋白过敏原结构与功能的研究进展[J].食品工业科技, 2013, 34 (21) :377-380.
[7]	Coates JB, Medeiros JS, Thanh VH, et al.Characterization of the subunits of beta-conglycinin[J].Archives of Biochemistry&Biophysics, 1985, 243 (1) :184.
[8]	郑树贵, 曹松屹, 孙泽威, 等.β-伴大豆球蛋白β亚基的纯化及免疫活性鉴定[J].大豆科学, 2009, 28 (2) :301-304.
[9]	闫慧丽.大豆主要过敏原Gly m Bd 28K Ig G结合表位的定位研究[D].郑州:河南工业大学, 2016.
[10]	Larralde O G, Martinez R, Camacho F, et al.Identification of hepatitis a virus mimotopes by phage display, antigenicity and immunogenicity[J].Journal of Virological Methods, 2007, 140 (1-2) :49-58.
[11]	Maruyama N, Adachi M, Takahashi K, et al.Crystal structures of recombinant and native soybeanβ-conglycininβhomotrimers[J].Eur J Biochem, 2001, 268 (12) :3595-3604.
[12]	卫会君, 司晓霞, 田怀东.β-伴大豆球蛋白β亚基缺失的大豆遗传资源的发掘[J].科技与创新, 2014 (4) :159.
[13]	杨廷亚, 王芳, 姜平, 等.应用噬菌体展示技术筛选兔出血症病毒抗原模拟表位[J].畜牧兽医学报, 2012, 43 (8) :1281-1286.
[14]	袁水林, 李欣, 陈红兵, 等.噬菌体展示技术定位主要食物过敏原表位的研究进展[J].食品工业科技, 2015, 36 (3) :379-384.
[15]	Wang W, Han J X, Wu Y J, et al.Simultaneous detection of eight food allergens using optical thin-film biosensor chips[J].J Agric Food Chem, 2011, 59 (13) :6889-6894.
[16]	张丹, 鲁会军, 金扩世, 等.猪乙型脑炎病毒E蛋白基因的分段克隆及原核表达[C].中国畜牧兽医学会2010年学术年会——中国兽医临床大会, 2010:303-305.