Comparison on bioactivities of supercritical CO2 fluid extracts and ethanol extracts from the fruit body of Ganoderma lucidum

The comparison on bioactivities of two sorts of extracts from the fruit body of Ganoderma lucidum obtained from supercritical CO2 fluid extraction (SFE-CO2) and ethanol extraction separately was investigated through applying a series of in vitro analytical methods including antitumor model, neurocyte anti-injury models and antioxidant model. The inhibition rate of supercritical CO2 fluid extracts (SFE-CO2E) against proliferation of tumor cell LNCaP, K562 and HepG2 was 78.37%, 87.09% and 78.85% respectively when inhibitory concentration reached 100μg/mL, which were higher than the inhibition effect of ethanol extracts (EtOHE) with 60.65%, 82.29% and 66.15% respectively. In vitro neurocyte anti-injury determination revealed that the repair effect of SFE-CO2E on cell PC12 (59.29%) was much more obvious compared with that of EtOHE (17.77%) at the concentration of 200μg/mL. The free radical scavenging experiments, however, demonstrated the scavenging activity of SFE-CO2E (29.24%, 39.43% and 17.05% respectively) was greatly lower than that of EtOHE (61.90%, 67.33% and 52.07% respectively) in analytical sequence of ·OH, H2O2 and DPPH· when effective concentration reached 800μg/mL. It was concluded that SFE-CO2 and ethanol extraction indicates various advantages on extracting different bioactive substances from the fruit body of Ganoderma lucidum.