Antioxidant activity of gelatin hydrolysates derived from salmon (Oncorhynchus keta) skin and their cytoprotective effect on oxidative injury of rat hepatocytes

doi:10.13386/j.issn1002-0306.2013.05.004

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Science and Technology of Food Industry > 2013 > (05): 113-117. > DOI: 10.13386/j.issn1002-0306.2013.05.004

Antioxidant activity of gelatin hydrolysates derived from salmon (Oncorhynchus keta) skin and their cytoprotective effect on oxidative injury of rat hepatocytes[J]. Science and Technology of Food Industry, 2013, (05): 113-117. DOI: 10.13386/j.issn1002-0306.2013.05.004

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Antioxidant activity of gelatin hydrolysates derived from salmon (Oncorhynchus keta) skin and their cytoprotective effect on oxidative injury of rat hepatocytes

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Received Date: September 02, 2012

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Abstract

Abstract

Seven chum salmon skin gelatin hydrolysates with degree of hydrolysis (DH) 4. 7%～13. 5% were prepared by three commercial proteases, Alcalase, papain or Neutrase, respectively. The antioxidant activity of gelatin hydrolysates was evaluated by the ability of scavenging free radicals. The results demonstrated that the antioxidant activity of the gelatin hydrolysates was gradually increased with the increase of degree of hydrolysis in a dose-dependent manner. Rat hepatocyte BRL cells were preincubated with gelatin hydrolysates (0.5, 1, 2mg/mL) and then were oxidative injuried by H2O2 (5mmol/L) . The cell viability, leakage of lactate dehydrogenase (LDH) , content of malondialdehyde (MDA) and GSH were determined by commercial kits. The results indicated that the cell viability was remarkably improved while the leakage of LDH and MDA formation were reduced significantly with the content of GSH almost unchanged. The statistical analysis of the results showed that seven gelatin hydrolysates played a protective role in H2O2-induced oxidative injury, and the cell viability was positively correlated with antioxidant activity of gelatin hydrolysates, while leakage of LDH and the content of MDA formed were negatively correlated with antioxidant activity of gelatin hydrolysates.
- gelatin hydrolysates;antioxidant activity;rat hepatocyte line (BRL cells) ;protective effect;correlation analysis;

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[1]	Moskovitz J, Yim KA, Choke PB.Free radicals and disease[J].Archives of Biochemistry and Biophysics, 2002, 397 (2) :354-359.
[2]	Stadtman ER.Protein oxidation and aging[J].Free Radical Research, 2006, 40 (12) :1250-1258.
[3]	Revilla E, Maria CS, Miramontes E, et al.Nutraceutical composition, antioxidant activity and hypocholesterolemic effect of a water-soluble enzymatic extract from rice bran[J].Food Research International, 2009, 42 (3) :387-393.
[4]	Suetsuna K, Ukeda H, Ochi H.Isolation and characterization of free radical scavenging activities peptides derived from casein[J].Journal of Nutritional Biochemistry, 2000, 11 (3) :128-131.
[5]	Sakanaka S, Tachibana Y.Active oxygen scavenging activity of egg-yolk protein hydrolysates and their effects on lipid oxidation in beef and tuna homogenates[J].Food Chemistry, 2006, 95 (2) :243-249.
[6]	Schrieber R, Gareis H.Gelatine handbook.Theory and industrial practice[M].Germany:Wiley-VCH, 2007.
[7]	Wu HC, Chen HM, Shiau CY.Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus) [J].Food Research International, 2003, 36 (9-10) :949-957.
[8]	Mendis E, Rajapakse N, Kim SK.Antioxidant properties of a radicals scavenging peptide purified from enzymatically prepared fish skin gelatin hydrolysate[J].Journal of Agriculture and Food Chemistry, 2005, 53 (3) :581-587.
[9]	Giménez B, Alemán A, Montero P, et al.Antioxidant and functional properties of gelatin hydrolysates obtained from skin of sole and squid[J].Food Chemistry, 2009, 114 (3) :976-983.
[10]	刘海英, 李丁, 李双祁, 等.斑点叉尾鮰鱼皮明胶水解方法的研究[J].食品工业科技, 2007, 28 (2) :176-178.
[11]	胡娟, 李琳, 胡松青.鱼鳞明胶酶解制备超氧阴离子清除活性肽的研究[J].食品工业科技, 2010, 31 (4) :293-295.
[12]	Jonglareonrak A, Benjakul S, Visessanguan W, et al.Isolationand characterisation of acid and pepsin-solubilised collagens from the skin of Brownstripe red snapper (Lutjanus vitta) [J].Food Chemistry, 2005, 93 (3) :475-484.
[13]	Edwards CA, O’Brien WD.Modified assay for determination of hydroxyproline in a tissue hydrolyzate[J].Clinica Chimica Acta, 1980, 104 (2) :161-167.
[14]	《食品中蛋白质的测定》[S].GB50010.5-2011.
[15]	Spellman D, Mcevoy E, O’Cuinn, et al.Proteinase and exopeptidase hydrolysis of whey protein:comparison of the TNBS, OPA and pH stat methods for quantification of degree of hydrolysis[J].International Dairy Journal, 2003, 13 (6) :447-453.
[16]	Nsimba R Y, KikuzakiH, Konishi Y.Antioxidant activity of various extracts and fractions of Chenopodium quinoa and Amaranthus spp.seeds[J].Food Chemistry, 2008, 106 (2) :760-766.
[17]	Li YH, Jiang B, Zhang T, et al.Antioxidant and free radical-scavenging activities of chickpea protein hydrolysate (CPH) [J].Food Chemistry, 2008, 106 (2) :444-450.
[18]	Tang XY, He ZY, Dai YF, et al.Peptide fractionation and free radical scavenging activity of zein hydrolysate[J].Journal of Agricultural and Food Chemistry, 2010, 58 (1) :587-593.
[19]	Mosmann T.Rapid colorimetric assay for cellular growth and survival:Application to proliferation and cytotoxicity assays[J].Journal of Immunological Methods, 1983, 65 (1-2) :55-63.
[20]	Wu HC, Chen HM, Shiau CY.Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus) [J].Food Research International, 2003, 36 (9-10) :949-957.
[21]	Mronga T, Stahnke T, Goldbaum O.Mitochondrial pathway is involved in hydrogen-peroxide-induced apoptotic cell death of oligodendrocytes[J].Glia, 2004, 46 (4) :446-455.
[22]	Ying HS, Gottron FJ, Choi DW.Assessment of cell viability in primary neuronal cultures.Current Protocols in Neuroscience[M].John Wiley&Sons, Inc., 2001.
[23]	Farmer EE, Davoine C.Reactive electrophile species[J].Current Opinion in Plant Biology, 2007, 10 (4) :380-386.
[24]	García-Nebot MJ, Cilla A, Alegría A, et al.Caseinophosphopeptides exert partial and site-specific cytoprotection against H2O2-induced oxidative stress in Caco-2cells[J].Food Chemistry, 2011, 129 (4) :1495-1503.

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