Immunostimulatory Activity of Tilapia-head Chondroitin Sulfate in RAW 264.7 Cells and Mice

HUANG Houpei; YUAN Yuan; WANG Zhuo; CHEN Jing; SONG Bingbing; ZHONG Saiyi

doi:10.13386/j.issn1002-0306.2023120214

Volume 45 Issue 23

Nov. 2024

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Science and Technology of Food Industry > 2024 > 45(23): 381-387. > DOI: 10.13386/j.issn1002-0306.2023120214

HUANG Houpei, YUAN Yuan, WANG Zhuo, et al. Immunostimulatory Activity of Tilapia-head Chondroitin Sulfate in RAW 264.7 Cells and Mice[J]. Science and Technology of Food Industry, 2024, 45(23): 381−387. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120214.

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Immunostimulatory Activity of Tilapia-head Chondroitin Sulfate in RAW 264.7 Cells and Mice

1.
Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China
2.
Shenzhen Institute, Guangdong Ocean University, Shenzhen 518063, China
3.
Collaborative Innovation Center of Seafood Deep Processing, Dalian 116034, China

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Received Date: December 20, 2023
Available Online: September 28, 2024

Graphical Abstract

Abstract

Abstract

In this study, RAW 264.7 cells and immunosuppressive models were used to investigate the immunostimulatory activity of tilapia-head chondroitin sulfate (CS) through in vitro and in vivo experiments. The results showed that tilapia-head chondroitin sulfate (CS) could induce the produce of NO, IL-1β, IL-6, IL-10 and TNF-α in RAW 264.7 cells. Cyclophosphamide (CTX) induced thymic atrophy in mice (thymic index 0.11%±0.01%), while thymic index was significantly up-regulated by low-dose CS (thymic index (0.17%±0.03%) and high-dose CS (thymic index (0.18%±0.02%) interventions (P<0.05). Meanwhile, CS significantly increased spleen index in CTX-induced mice (P<0.05), promoted villus length and V/C value, with high-dose CS significantly increased villus length (330.92±21.55) μm (P<0.05). Furthermore, CS enhanced the secretion of serum sIgA in CTX-induced mice, indicating that CS could improve intestinal barrier damage and immune function in immunosuppressive mice. Therefore, this study provides a theoretical basis for the intestinal mucosal immune regulation of tilapia chondroitin sulfate, and suggests that tilapia-head chondroitin sulfate (CS) is a potential immunoregulatory polysaccharide that could replace shark chondroitin sulfate, potentially serving as a promising material for functional foods aimed at intervening in immune-related diseases.
- tilapia,
- chondroitin sulfate,
- immunostimulatory activity,
- immunosuppression,
- intestinal barrier,
- RAW 264.7

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