Simultaneous Determination of 8 Mycotoxins in Aquatic Products by Liquid Chromatography-Tandem Mass Spectrometry Coupled with Immunoaffinity Column Clean-up

LI Yifeng; ZHANG Qiuyun; YANG Hongsheng; ZHU Xiaohua; SHEN Meifang; CHEN Huimin

doi:10.13386/j.issn1002-0306.2022050233

Volume 44 Issue 7

Mar. 2023

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Science and Technology of Food Industry > 2023 > 44(7): 294-300. > DOI: 10.13386/j.issn1002-0306.2022050233

LI Yifeng, ZHANG Qiuyun, YANG Hongsheng, et al. Simultaneous Determination of 8 Mycotoxins in Aquatic Products by Liquid Chromatography-Tandem Mass Spectrometry Coupled with Immunoaffinity Column Clean-up[J]. Science and Technology of Food Industry, 2023, 44(7): 294−300. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022050233.

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Simultaneous Determination of 8 Mycotoxins in Aquatic Products by Liquid Chromatography-Tandem Mass Spectrometry Coupled with Immunoaffinity Column Clean-up

1.
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
2.
Freshwater Fishery Research Institute of Jiangsu Province, Nanjing 210017, China
3.
Jiangsu Aquatic Product Quality Inspection Center, Nanjing 210017, China
4.
Shanghai AB Sciex Analytical Instrument Trading Co., Ltd., Shanghai 200335, China

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Received Date: May 18, 2022
Available Online: February 05, 2023

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Abstract

Abstract

Objective: A liquid chromatography-tandem mass spectrometry coupled with immunoaffinity column clean-up method was established for the determination of 8 mycotoxins in aquatic products. Methods: The samples were extracted with acetonitrile water (84:16, V:V), and purified by multifunctional immunoaffinity columns. The chromatographic column was Agilent Proshell 120 SB·C₁₈ column (2.1 mm×100 mm, 2.7 μm). Mobile phase was methanol and 5 mmol/L ammonium acetate (containing 0.1% formic acid), gradient elution. The flow rate was 0.4 mL/min, and the column temperature was 40 ℃. The electrospray was operated in both positive and negative mode at the same time, and the samples were detected by multiple reaction monitoring mode (MRM). Results: The linear relations of the eight mycotoxins were good within the concentration range of 1.0~50.0 ng/mL (R²>0.992). The limits of detection were between 0.05~0.50 μg/kg, and the limits of quantification were between 0.17~1.65 μg/kg. The average recoveries were 75.6%~106.3%, and the relative standard deviations (RSDs) were 3.5%~9.3%. Conclusion: The method is simple, sensitive and accurate for the rapid analysis of mycotoxin residues in aquatic products.
- mycotoxins,
- aquatic products,
- liquid chromatography-tandem mass spectrometry,
- immunoaffinity column

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References (31)

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