豆腐柴叶总黄酮不同溶剂级分抗氧化活性分析

熊双丽; 马楠; 廖婷婷; 薛朝云

doi:10.13386/j.issn1002-0306.2017.18.004

豆腐柴叶总黄酮不同溶剂级分抗氧化活性分析

西南科技大学生命科学与工程学院
四川省生物质资源利用与改性工程技术研究中心
江油市春雨生态农业科技有限公司

基金项目:

四川省生物质资源利用与改性工程技术研究中心专职科研创新团队建设基金项目(14tdgc04)；四川省科技厅项目(2017NFP0179)；

详细信息

作者简介:
熊双丽 (1977-) , 女, 博士, 教授, 研究方向:功能性食品加工与安全, E-mail:372364129@qq.com。;

中图分类号: TS201.2
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出版历程
- 收稿日期: 2017-02-22

Antioxidant activity of flavone with different solvent extracts from Premna microphylla Turcz leaves

School of Life Science and Engineering, Southwest University of Science and Technology
Engineering Research Center for Biomass Resource Utilization and Modification of Sichuan Province
Jiangyou Chunyu Ecological Agricultural Science and Technology Company

摘要

摘要: 采用1,1-二苯基-2-三硝基苯肼自由基、羟自由基、超氧阴离子、还原力、总抗等体外抗氧化活性模型和抗氧化活性综合评价指数,分析四川江油地区不同溶剂萃取的豆腐柴黄酮级分（乙醇、乙酸乙酯、正丁醇、氯仿、水）的抗氧化活性。结果显示,不同浓度乙醇提取总黄酮的1,1-二苯基-2-三硝基苯肼自由基清除活性最高。1,1-二苯基-2-三硝基苯肼自由基清除率、羟基自由基清除率、ABTS阳离子自由基清除率、总抗和还原力皆随乙醇浓度的升高而呈现先增强后减弱的趋势,超氧阴离子自由基清除率却随乙醇浓度增加一直降低。综合评价指数显示不同浓度乙醇抗氧化活性依次为75%醇提物>65%醇提物>55%醇提物>85%醇提物>95%醇提物。乙酸乙酯层萃取物的1,1-二苯基-2-三硝基苯肼自由基清除率、总抗、还原力及ABTS阳离子自由基清除率要优于其它各萃取层。正丁醇层萃取物的超氧阴离子自由基清除率要优于其它各萃取层。乙酸乙酯层抗氧化活性最佳。豆腐柴叶总黄酮有较好的抗氧化活性,可作为抗氧化剂或者健康食品原料开发。
- 豆腐柴 /
- 黄酮 /
- 分级分离 /
- 抗氧化
Abstract: The article mainly evaluated the antioxidant ability of total flavonoids in various extracts ( ethyl alcohol, ethyl acetate, normal butyl alcohol, chloroform fraction and water) from Premna microphylla Turcz leaves in Jiangyou area including DPPH radical, ABTS+· radical, hydroxyl radical, superoxide anion radical scavenging effect, reducing power, ferrous ions chelating activities and total antioxidant capacity ( FRAP) . The free radical scavenging activity of total flavonoids extracted by different concentrations of ethanol was highest in DPPH. The free radical scavenging activity ( DPPH radical, ·OH, ABTS+·) , total antioxidant capacity ( FRAP) and reducing power were dependent on the concentration of alcohol increasing first and then decreasing, while the superoxide anion radical scavenging activity was contrary to the increase of alcohol concentration. The comprehensive evaluation index ( APC) of tested samples was decreased in the following order: 75% ethanol extract > 65%ethanol extract > 55% ethanol extract > 85% ethanol extract > 95% ethanol extract. The DPPH radical scavenging activity, FRAP value and reducing power were higher than those of other solvent extracts.The superoxide anion radical scavenging rate of ethyl acetate extract was stronger than those of other solvent extracts. The antioxidant capacity of ethyl acetate extract was strongest according to the APC results.The flavone from leaves of Premna microphylla Turcz has strong antioxidant activity and can be used as antioxidant or raw materials of healthy food.
- Premna microphylla Turcz /
- flavone /
- fractionation /
- antioxidant activity

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