Study on the antioxidant activity of isochlorogenic acid A
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摘要: 从忍冬根药材中提取异绿原酸A,采用DPPH法、水杨酸比色法、邻苯三酚自氧化法及还原力测定法来进行体外抗氧化实验,利用皮肤荧光斑马鱼模型研究异绿原酸的体内抗氧化活性。结果表明,在0.5~10μg/m L范围内,异绿原酸A对DPPH和羟基自由基表现出了较好的清除作用,清除率最高分别达97.04%和60.01%;而异绿原酸A在5~200μg/m L范围内超氧自由基清除率最高仅达到28.04%;还原力测定OD值在0.5~100μg/m L范围内最高达0.98,说明异绿原酸A的还原能力强;异绿原酸A在皮肤荧光斑马鱼体内抗氧化效果良好,在浓度为25μg/m L时,相对抗氧化能力可达到60.4%。异绿原酸A对自由基有较好的清除能力,有良好的体内体外抗氧化活性。Abstract: To investigate the antioxidant activity of isochlorogenic acid A in vitro and in vivo. Isochlorogenic acid A from honeysuckle herbs was extracted.The antioxidant activities of isochlorogenic acid A was evaluated by spectrophotometry on its ability of scavenging ·OH, O-2·, DPPH· and the reduction force.The zebrafish was used to test the antioxidant activities of isochlorogenic acid A in vivo.The result showed that the isochlorogenic acid A had well scavenging activity to DPPH, ·OH and O-2·in the range of experimental concentration, the highest scavenging of DPPH and OH were reached 97.04%, 60.01%respectively.However, the highest scavenging of O-2· reached just 28.04%. Determination of OD in reduction ability of isochlorogenic acid A up to 0.98, illustrating that isochlorogenic acid A had a highly reduction ability. In vivo experiments in zebrafish showed that the antioxidant capacities were reached to 60.4%, when the concentration of sample were 25 μg/m L. So the isochlorogenic acid A showed effective antioxidative action both in vitro and in vivo.
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Keywords:
- isochlorogenic acid A /
- zebrafish /
- antioxidant activities /
- free radical
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