甜茶黄酮回收工艺及抗氧化性研究

刘程程; 张彩云; 郑奎玲; 陈显刚; 廖莉玲

doi:10.13386/j.issn1002-0306.2017.09.030

甜茶黄酮回收工艺及抗氧化性研究

贵州师范大学化学与材料科学学院
贵阳护理职业学院
贵州省健康茶叶科技有限公司

基金项目:

贵州省科学技术厅中药现代化攻关项目(黔科合ZY字【2012】3012号)；贵阳市科技局现代药业计划项目(筑科合同[2012204]号)；

详细信息

作者简介:
刘程程 (1984-) , 女, 硕士, 讲师, 从事分析化学研究, E-mail:lccangel@163.com。;

廖莉玲 (1963-) , 女, 硕士, 教授, 从事天然产物方面的研究, E-mail:lll6383@163.com。;

中图分类号: TS218
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出版历程
- 收稿日期: 2016-10-27

Study on recycling technology and antioxidant activities of total flavones of sweet tea

School of Chemistry and Material Science, Guizhou Normal University
Guiyang Nursing Vocational College
Guizhou Province Health Tea Science and Technology Limited Company

摘要

摘要: 在原有生产甜茶素工艺的基础上,探究了总黄酮的回收工艺;同时以维生素C为参照对甜茶总黄酮清除·OH和DPPH·的能力进行了检测。按照工厂的生产工艺在实验室模拟实验,采用紫外可见分光光度法测定各阶段的总黄酮含量。结果表明:采用75%的乙醇洗脱聚酰胺树脂上的黄酮,黄酮的洗脱率为91.33%,洗脱液旋干得到的总黄酮的含量为36.30%。75%的乙醇洗脱液经过FL-3树脂进一步纯化,旋干所得产品总黄酮含量可达62.70%,得率为1%（以甜茶叶计）。对纯化后的甜茶总黄酮样进行紫外扫描光谱和高效液相色谱分析,初步判断样品中含有芦丁、槲皮素。由甜茶总黄酮的抗氧化活性的实验得知,甜茶总黄酮的抗氧化活性很高;清除·OH的能力:粗黄酮>精黄酮>维生素C;在清除DPPH·的能力方面,当浓度大于0.08 mg/m L以后,粗黄酮、精黄酮和维生素C的清除能力区别不大;在低于0.08 mg/m L时,实验结果同样是粗黄酮>精黄酮>维生素C。
- 甜茶 /
- 总黄酮 /
- 抗氧化性 /
- 回收工艺
Abstract: The recycling technology of flavonoids was explored meanwhile unchanging original production of rubusoside. The removal capacity of the purified and unpurified flavonoids of sweet tea on·OH, DPPH·were studied by vitamin C as reference.Experiments were conducted in the laboratory according to production process in the factories and the contents of the total flavone in different parts were determined by using UV method.The results showed that the elution rate of flavonoids from the polyamide resin was up to 91.33% by 75% ethanol as elution liquid and content of flavonoids was up to 36.3%.75% ethanol desorption solution was purified by FL-3 resin further, the content of flavonoids in the final product was up to 62.70%, the yield was 1%.There were rutin and quercetin in the final flavonoids product by UV and HPLC analysis.Flavonoids compounds in sweet tea had good oxidation resistance.·OH scavenging ability were in order: unpurified flavonoids > purified flavonoids >vitamin C.DPPH· scavenging ability of unpurified flavonoids, purified flavonoids and vitamin C were almost the same when the concentration was more than 0.08 mg/m L, ability of DPPH· scavenging were in order: unpurified flavonoids > purified flavonoids > vitamin C when concentration is less than 0.08 mg/m L.
- sweet tea /
- the total flavone /
- antioxidant activity /
- the recycling process

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