Study on bioavailability of protein hydrolysate- citrate calcium prepared from shrimp shell
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摘要: 目的:评价从虾壳中制取的蛋白水解物-柠檬酸钙复合钙粉（PHC-Ca）的生物利用率。方法:选择4周龄体重无显著性差异的SD大鼠60只,随机分为6组,在自由进食低钙饲料的基础上分别以PHC-Ca、碳酸钙（Ca CO3）、柠檬酸钙、葡萄糖酸钙以及虾壳细粉灌胃饲养,每日灌胃剂量为66.5 mg Ca/（kg·d）（以钙剂量及大鼠体重为计算指标）。喂养至4周末进行3 d代谢实验,测定钙表观吸收率、储存率。继续喂养至8周末,观察大鼠体格增长并测定血清碱性磷酸酶、骨钙素水平以及股骨相关指标。结果:低钙对照组大鼠各项体生长指标均显著低于除碳酸钙组外的其他组（p<0.05）;PHC-Ca能显著提高大鼠钙表观吸收率、钙储存率,且能降低血清碱性磷酸酶、骨钙素水平（p<0.05）;摄食PHC-Ca、葡萄糖酸钙的大鼠,其血清钙水平无显著性差异（p>0.05）且高于其他组;PHC-Ca组大鼠股骨钙、股骨长、股骨干重指标高于葡萄糖酸钙组、柠檬酸钙组、虾壳粉组（p<0.05）。结论:从虾壳中制取的PHC-Ca有促进钙吸收的效果,其生物利用率优于相同钙含量的柠檬酸钙以及虾壳粉,可作为一种良好的膳食钙补充剂。
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关键词:
- 虾壳 /
- 蛋白水解物-柠檬酸钙 /
- 生物利用率
Abstract: Objective: To evaluate bioavailability of protein hydrolysate- citrate calcium( PHC- Ca) from shrimp shell.Method: 4 weeks SD rats were randomly divided into 6 groups with 10 rats in each,and then gavaged with PHC- Ca,Ca CO3,calcium citrate,calcium gluconate,shrimp shell powder based on low calcium diet and low calcium control diet for 8 weeks.The calcium gavage dose to rats was 66.5 mg Ca /( kg·d).Calcium metabolic test was conducted by the end of the forth week.Moreover,the index related to serum and bone were assayed by the end of the 8th week.Result: The rats' growth index in low calcium was lower than other group except Ca CO3.The calcium absorption rate of PHC- Ca group was remarkable high than other groups except low calcium control.Furthermore,the level of ALP,BGP,in serum indicated that PHC- Ca improved calcium absorption in serum level significantly( p < 0.05). Moreover,the bone index showed the bioavailability in PHC- Ca group( p < 0.05).Conclusion: PHC- Ca prepared from shrimp shell could be promote calcium absorption,its bioavailability was better than same calcium content of calcium citrate and shrimp shell powder,which could be used as sources of dietary calcium supplement. -
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