SPSS软件在黑曲霉产异淀粉酶培养基优化中的应用

张敏; 杨红; 母荣飞; 吴茜茜

doi:10.13386/j.issn1002-0306.2014.24.037

SPSS软件在黑曲霉产异淀粉酶培养基优化中的应用

合肥学院生物与环境工程系

基金项目:

安徽省教育厅重点项目(KJ2012A246)；合肥学院生物工程院级重点学科；合肥学院发酵工艺重点实验室；

详细信息

作者简介:
张敏 (1967-) , 博士, 副教授, 研究方向:微生物酶学。;

吴茜茜 (1974-) , 女, 硕士, 教授, 研究方向:发酵工程和酶工程。;

中图分类号: TQ925.1
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出版历程
- 收稿日期: 2014-07-06

Application of SPSS on optimization of culture medium of isoamylase from Asperigillus niger

Department of Biological and Environmental Engineering, Hefei University

摘要

摘要: 在固态发酵条件下,研究了碳源、氮源对黑曲霉（Aspergillus niger）淀粉酶系的影响,探索糖化酶、酸性α-淀粉酶及异淀粉酶的分泌规律;采用正交实验进一步优化产酶培养基,其方差分析和S-N-K法分析结果表明:黑曲霉产异淀粉酶的优化培养基分别为麸皮7.5g,葡萄糖0.06g,支链淀粉0.24g,酵母粉0.5g,营养盐8m L;在此基础上,缩短了异淀粉酶最高酶活形成时间,同时糖化酶、酸性α-淀粉酶最高酶活分别降低了36.7%和166%,这为异淀粉酶分离纯化提供了基础。
- Aspergillus niger /
- 异淀粉酶 /
- 酸性α-淀粉酶 /
- 糖化酶 /
- 固态发酵
Abstract: The effects of different carbons and nitrogens on characteristics of starch degrading enzyme from Aspergillus niger in solid state fermentation were studied. And synthenic regularities of glucoamylase, acidic α-amylase and isoamylase were also explored. Orthogonal experiment was used to optimize medium compositions further, and its results showed that the optimial medium components for isoamylase were composed of bran 7.5g, glucose 0.06 g, amylopectin 0.24 g, yeast powder 0.5g and nutritive salt solution 8m L based on analysis of variance (ANOVA) and S-N-K method with SPSS software. Under the above medium, formation time of highest isoamylase activity was shorten, while the highest activity of glucoamylase and acidα-amylase were decreased by 36.7% and 166%, respectively. It was benefit for further isolation and purification of isoamlyase.
- Aspergillus niger /
- isoamylase /
- acidic α-amylase /
- glucoamylase /
- solid-state fermentation

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