Screening and identification of an antagonistic yeast and its control efficacy to postharvest decay of grapes
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摘要: 从桑葚中筛选得到一株具有潜在生防效力的酵母,经分子生物学鉴定及生物化学检验,确定其为酿酒酵母。该酵母对葡萄采后黑曲霉病具有显著的抑制效果,随着酵母菌浓度的增高,葡萄腐烂率逐步降低;当酿酒酵母浓度为1×108cfu/mL时,对葡萄黑曲霉病的抑制效果最好。在20℃和4℃条件下,该酵母在葡萄伤口处能快速繁殖,能显著抑制葡萄自然腐烂率,而对其贮藏品质影响不显著。Abstract: A strain of potential antagonistic yeast was isolated from mulberry fruits, which showed the biocontrol efficacy against postharvest decay of grapes. By molecular biological identification and biochemical examination, the antagonistic strain was identified as Saccharomyces cerevisiae. The antagonist showed significant biological control efficacy against postharvest decay of grapes caused by Aspergillus niger. When the concentration of the antagonist was higher, the disease incidence was lower. The concentration of S. cerevisiae was 1×108cfu/mL, the inhibition effect of grapes diseased by Aspergillus niger was best. Rapid growth of the antagonists in wounds was observed at 20℃ and 4℃. S. cerevisiae significantly reduced the natural development of decay and did not have significant effect on postharvest natural quality.
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Keywords:
- screening /
- identification /
- Saccharomyces cerevisiae /
- grapes /
- postharvest decay /
- biocontrol
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