Study on stability of the yellow pigment from a new marine actinomycete and 16S rDNA analysis of the strain
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摘要: 对一株分离自威海海域的产黄色素的海洋放线菌新菌株MA01进行了色素提取方法及其稳定性的研究。测定其最大吸收波长,考察了pH、光照、温度、金属离子、氧化剂和还原剂、防腐剂等因素对黄色素稳定性的影响。此外,对菌株MA01进行分子鉴定,利用PCR技术扩增其16S rDNA序列,并构建了系统进化树。实验结果表明,该黄色素在300nm处有最大吸收峰,超声波法提取色素优于溶剂法。该黄色素为水溶性色素,易溶于甲醇和乙醇,在弱酸性、中性和碱性条件下稳定,温度小于60℃时热稳定性良好,对紫外光和可见光照射稳定性良好,基本不受金属离子K+、Na+、Mg2+、Mn2+、Ca2+影响,防腐剂食盐和蔗糖对其均无不良影响。该黄色素不耐氧化剂,还原剂也会降低其稳定性。16S rDNA序列分析和系统进化树显示菌株MA01与多株Streptomyces sp.的16S rDNA具有99%的相似性,初步鉴定菌株MA01是链霉菌属Streptomyces sp.的一种。菌株MA01具有较高的研究和开发价值。Abstract: Extraction conditions, basic properties and the stability of the yellow pigment produced by a new marine actinomycete strain MA01, which was isolated from Weihai sea area, were investigated in this paper.The maximum absorption wavelength was measured, and the stability of the pigment was assessed under the conditions as following:the different pH values, UV, fluoresnt lighting, the different temperatures, some kinds of ions, oxidant/reducer and preservatives. In addition, the 16S rDNA fragment of MA01 was amplified by PCR for molecular characterization, and the phylogenetic tree was constructed. Results showed that the maximum absorption wavelength of this yellow pigment was 300nm. The pigment was extracted better by ultrasonic extraction method than by solvent method. This yellow pigment was classified as a kind of water-soluble pigment, and is soluble in methanol and ethanol. Furthermore, it was stable at the temperature below 60℃ and under the neutral and alkaline conditions, but sensitive to oxidant, while had a good resistance to UV and light.Some metal ions (e.g. K+, Na+, Mg2+, Mn2+, Ca2+) and common preservatives (e.g. salt and sucrose) did not affect the stability of the pigment. Phylogenetic tree analysis of 16S rDNA sequence indicated that the similarity between MA01 and multiple strains of Streptomyces sp.was higher than 99%. Therefore, MA01 was identified as a new strain belonging to Streptomyces sp., and had good value for research.
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Keywords:
- marine actinomycete /
- yellow pigment /
- stability /
- 16S rDNA /
- Streptomyces sp.
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[1] 苏龙, 詹萍, 陈旭健, 等.微生物产天然红色素提取技术[J].玉林师范学院学报:自然科学版, 2008, 29 (3) :84-86. [2] 郑亚军, 苏冰霞, 陈卫军, 等.槟榔红色素的抗氧化活性[J].热带作物学报, 2009, 30 (6) :881-884. [3] 唐秋琳, 赵海, 戚天胜.天然食用黄色素研究进展[J].中国食品添加剂, 2006 (2) :68-73. [4] 邱斌, 陈卫平, 王青.栀子黄色素稳定性研究[J].食品研究与开发, 2009, 30 (9) :190-192. [5] 闫绍辉, 张德权.栀子色素研究进展与开发[J].食品研究与开发, 2000, 21 (6) :28-31. [6] 陈合, 李世玉, 舒国伟, 等.黄姜色素提取条件的研究[J].陕西科技大学学报, 2009, 27 (4) :52-54, 72. [7] Korutla L, Cheung JY, Medelsohn J, et al.Inhibition of ligandinduced activation of epidermal growth factor receptor tyrosine phosporylation by curcumin[J].Carcinogenesis, 1995, 16 (8) :1471-1475.
[8] Watanabe T, Terabe S.Analysis of natural food pigments by capillary electrophorese[J].J Chomatography A, 2000, 880:311-322.
[9] 许钢.天然红花黄色素稳定性研究[J].食品工业科技, 2000, 21 (1) :16-18. [10] 姚艾东.玉米黄色素的提取及工艺研究[J].食品工业科技, 2001, 22 (4) :32-34. [11] 张宏宸, 沈萼芮, 谭毅.玉米黄素性质和应用研究[J].酿酒, 2009, 36 (3) :54-55. [12] 钟岩, 李泽鸿, 周丹.桔皮黄色素提取方法的研究[J].北方园艺, 2008 (3) :30-31. [13] 刘铮.天然南瓜黄色素的性能研究[J].广西科学, 2004, 11 (1) :58-60. [14] 廖振林, 徐丽华, 蔡淑娇, 等.一株新的海洋放线菌黄色素的提取与稳定性研究[J].食品科技, 2008 (10) :170-172, 177. [15] 施思, 胡承, 张文学.一株放线菌产棕色素初探及其16S rDNA序列分析[J].食品工业科技, 2010, 31 (10) :239-240, 250. [16] 魏力, 方加玮, 周俊初, 等.一株海洋细菌的初步鉴定及其产黑色素相关新基因 (簇) 的分离[J].微生物学通报, 2007, 34 (6) :1118-1122. [17] 侯竹美, 王淑军, 赵方庆, 等.胶州湾产蓝色素海洋链霉菌的初步研究[J].海洋科学, 2007, 31 (5) :39-44, 57. [18] Maskey RP, Kock I, Helmke E, et al.Isolation and structure determination of phenazostatin D, a new phenazine from a marine actinomycete isolate Pseudonocardia sp.B6273[J].Zeitschrift Für Naturforschung, 2003, 58b (7) :692-694.
[19] Solano F, García E, Perez D, et al.Isolation and characterization of strain MMB-1 (CECT 4803) , a novel melanogenic marine bacterium[J].Applied and Environmental Microbiology, 1997, 63 (9) :3499-3506.
[20] 张晔, 赵晶晶, 王锴, 等.超声波辅助提取辣椒红色素[J].食品研究与开发, 2011, 32 (1) :71-74. [21] 张秋荣, 单丽红, 杜斌, 等.超声波提取玉米黄色素的工艺研究[J].郑州大学学报:工学版, 2005, 26 (2) :100-112. [22] 谢凤霞, 邱祖民, 涂盛辉, 等.浸提法与超声波提取法提取栀子黄色素的比较[J].南昌大学学报:理科版, 2005, 29 (3) :278-281. [23] 杜宗军, 赵苑, 李美菊, 等.青岛近海琼胶降解细菌的筛选和多样性分析[J].中国海洋大学学报:自然科学版, 2007, 37 (2) :277-282.
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