产碱性脂肪酶菌株的筛选及酶学性质研究

钟桂芳; 袁海晓; 史文婷; 魏东芝; 杨雪鹏

doi:10.13386/j.issn1002-0306.2013.20.026

产碱性脂肪酶菌株的筛选及酶学性质研究

郑州轻工业学院食品与生物工程学院

基金项目:

河南省教育厅科技攻关项目(102102110044)；

详细信息

作者简介:
钟桂芳 (1973-) , 女, 讲师, 主要从事酶工程方面的研究。;

中图分类号: TQ925.6
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- 文章访问数: 161
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出版历程
- 收稿日期: 2013-03-28

Screening of lipase producing strains and studying on enzymatic properties of lipase

Food and Bioengineering College, Zhengzhou University of Light Industry

摘要

摘要: 从富油土壤中筛选出一株耐热碱性脂肪酶产生菌X-5,经菌落形态观察、生理生化实验和16S rDNA序列分析,初步鉴定为假单胞菌。采用单因素实验研究其生长条件,研究表明该菌株最适生长温度为34℃,最适生长pH为8.0,6²6h为对数生长期,26³8h为稳定期,38h开始进入衰亡期;酶学性质研究表明,该酶最适反应温度为60℃,最适反应pH为8.5,70℃保温1h仍保留60%的酶活,具有良好的耐热性。K+、Na+对酶有激活作用,Ca2+、Mg2+、Zn2+、Fe2+、Cu2+、Mn2+对酶有不同程度地抑制作用。
- 碱性脂肪酶 /
- 筛选 /
- 酶学性质
Abstract: The X-5 strain producing thermotolerant alkaline lipase was isolated from rich oil soil. It was identified as pseudomonas sp. according to morphologic observation, physiology and biochemistry experiments and 16S rDNA sequence analysis. The optimal growing conditions were studied by single-factor experiment. The result showed that the following were the optimal growing conditions of X-5:the optimal growing temperature was34℃ and the optimal growing pH was 8.0. The growing curves showed that logarithmic phase started after 6hours. It was in the stationary phase from 26³8 hours, then after 38 hours decline phase started. The study about enzymatic properties showed that the optimal temperature and pH were 60℃ and 8.5 respectively. It remained 60 percent enzymatic activity in 70℃ for one hour. K+、Na+could improved enzymatic activity, however, other ions such as Ca2+, Mg2+, Zn2+, Fe2+, Cu2+and Mn2+ had opposite influence.
- alkaline lipase /
- screening /
- enzymatic properties

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参考文献(13)

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