抗性淀粉的体外消化及结构变化的研究
详细信息Study on in vitro digestion and structure change of resistant starch
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摘要: 以玉米淀粉和增抗玉米淀粉为原料进行体外消化实验,建立了消化动力学模型,利用X-射线衍射、差示扫描量热、傅里叶红外变换等手段,对抗性淀粉不同时间段的消化物进行分析。结果表明:玉米淀粉在2h内几乎完全水解,消化速率常数为2.32h-1,高于抗性淀粉的1.13h-1;抗性淀粉在0～2h内,结晶度增加,焓值增加,表明此时消化过程以水解动力学为主,2h后,结晶度稍有下降,整体呈现一个无规律的变化过程;此时为直链淀粉重新排列的过程,链间通过氢键连接,形成的聚合物能抵抗淀粉酶的水解作用,整个消化过程为水解动力学和老化动力学相互竞争的过程。Abstract: To prepare corn resistant starch by pullulanase, and establish digestion kinetic model compared with corn starch.Digestion of resistant starch through different time was investigated by X- ray diffraction, differential scanning calorimetry, Fourier transform infrared.The results showed that corn starch was almost hydrolyzed in 2h, hydrolysis rate constant was 2.32h - 1 , higher than resistant starch ( 1.13h - 1 ) .Both crystallinity and enthalpy were increased, which showed that proceed mainly was hydrolysis kinetic.After 2h, crystallinity decreased but showed a disordered change process, amylose was rearrangement and linked by hydrogen bond, the formed polymer can resist amylase hydrolysis.The digestion showed a competition between the kinetics of enzyme hydrolysis and the kinetics of amylose retrogradation.
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