黄芩总黄酮超声提取工艺研究
详细信息Study on ultrasonic extraction techniques of total flavones from Scutellaria baicalensis Georgi
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摘要: 目的:优选超声波法提取黄芩总黄酮的工艺条件,为工业化生产中提取黄芩总黄酮提供参考。方法:采用超声波法提取黄芩中总黄酮,通过正交实验确定最佳提取工艺。结果:最佳提取工艺为超声温度60℃,液固比14∶1(mL/g),甲醇浓度80%,超声时间50min,此条件下的总黄酮得率为7.06%。且四个因素对黄芩总黄酮提取的影响程度依次如下:液固比>提取温度>提取时间>溶剂浓度。结论:该工艺提取简便、快速、得率高,为工业应用提供了精确有效的参考标准,为广泛开发利用黄芩资源提供了良好的参考价值。Abstract: Objective: The ultrasonic extraction technique of total flavones was optimized in order to provide a reference for extracting total flavones from Scutellaria baicalensis Georgi. Methods: On the basis of single factor experiments, the orthogonal experiment was used in the optimization of ultrasonic extraction technological parameters for obtaining good yield rate of total flavones.Results: The optimum conditions were as follows: the ratio of liquid to solid 14 ∶ 1 ( mL/g ) , emperature 60℃, methanol concentration 80% , ultrasonic time 50min. Under this condition, the yield of total flavones was 7.06%.And the effect of the four factors on the yield rate was as follows: liquid-to-solid ratio > extraction temperature > extraction time > solvent concentration. Conclusion: This ultrasonic extraction technique was simple and rapid, which had good yield rate.Furthermore, this study offered not only an accurate and effective reference standard for industrial applications, but also a good reference value for widely exploitation and utilization of Scutellaria baicalensis resource.
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[1] Kowalczyk E, Krzesinski P, Kura M.Pharmacological effects of flavonoids from Scutellaria baicalensis[J].Przegl Lek, 2006, 63 (2) :95-96.
[2] 刘金霞, 王立平, 徐晓惠.黄芩茎叶总黄酮对W256瘤细胞引起大鼠骨侵袭抑制作用[J].中国药理学通报, 2005, 21 (1) :125-126. [3] Nguyen Xuan Nhiena, Phan Van Kiem, Chau Van Minh.A potential inhibitor of rat aortic vascular smooth muscle cell from the pollen of typha angustata[J].Archives of Pharmacal Research, 2010, 33 (12) :1937-1942.
[4] Wu Sujuan, Sun Ailing, Liu Renmin.Separation and purification of baicalin and wogonoside from the Chinese medicinal plant scutellaria baicalensis georgi by high-speed counter-current chromatography[J].Journal of Chromatography A, 2005, 1066 (12) :243-247.
[5] Chi Ruan, Zhou Fang, Huang Kun.Separation of baicalinfrom scutellaria baicalensis georgi with polyamide[J].Cent South Univ Technol, 2008, 15:606-611.
[6] 曾超珍, 刘志祥, 韩磊.黄芩总黄酮提取技术及其抑菌活性研究[J].时珍国医国药, 2009, 20 (6) :1342-1343. [7] 张春云, 谢伟刚, 张雅丽, 等.黄芩总黄酮的含量测定及其提取工艺优化[J].光谱实验室, 2011, 28 (3) :1253-125. [8] 曲中堂, 王光利, 项昭保, 等.青果总黄酮的含量测定及超声提取工艺研究[J].食品工业科技, 2012, 33 (7) :291-294.
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