斑点叉尾鮰鱼皮胶原蛋白的理化特征研究
详细信息Physical and chemical properties of collagen from the skin of channel catfish
-
摘要: 选用斑点叉尾鮰（Ictalurus punctatus）鱼皮为材料,用乙酸和乙酸-胃蛋白酶,分别提取鱼皮中的酸溶性胶原蛋白（acid-solubilise collagen,ASC）和酶溶性胶原蛋白（pepsin-solubilise collagen,PSC）,并对其理化性质特征进行研究。研究发现,提取得到的ASC纯度高达93.11%,PSC纯度高达93.46%;紫外吸收分析表明,ASC和PSC的吸收峰值均在233nm处;蛋白图谱中两种胶原蛋白均由两种不同的α链（α1）2α2组成,具备I型胶原蛋白的特征;ASC和PSC的傅里叶红外图谱相似,具有完整的三螺旋结构;ASC的变性温度为34.2℃,PSC的变性温度为33.9℃。Abstract: The acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted from skin of channel catfish by the method of acid and acid-pepsin, and their physical and chemical characterization were analysed.The study indicated that the ASC had high purity of 93.11% and PSC of 93.46%, the analysis of UV spectra of ASC and PSC showed that the maximum absorption was obtained at 233nm.Both ASC and PSC consisted of two different α-chains (α 1) 2 α 2 were characterized as type I.Fourier transform infrared (FTIR) spectra of both ASC and PSC were almost similar with whole triple-helical structure.Transition temperature of ASC and PSC was 34.2℃ and 33.9℃.
-
[1] 汤克勇.胶原物理与化学[M].北京:北京科学出版社, 2012:161-168. [2] Kanokwan Matmaroh, Soottawat Benjakula, Thummanoon Prodpran, et al.Characteristics of acid soluble collagen and pepsin soluble collagen from scale of spotted golden goatfish (Parupeneus heptacanthus) [J].Food Chemistry, 2011, 129:1179-1186.
[3] Prabjeet Singh, Soottawat Benjakul, Sajid Maqsood, et al.Isolation and characterisation of collagen extracted from the skin of stripedcatfish (Pangasianodon hypophthalmus) [J].Food Chemistry, 2011, 124:97-105.
[4] Zunying Liu, Alexandra CM Oliveira, YinCheng Su.Purification and characterization of pepsin-solubilised collagen from skin and connective tissue of giant red sea cucumber (Parastichopus californicus) [J].Journal of Agricultural and Food Chemistry, 2010, 58 (2) :1270-1274.
[5] 王卫东, 李超, 孙月娥.鱼皮明胶的制备、特性及应用[J].食品科学, 2009, 30 (20) :484-485. [6] 易继兵, 李八方, 赵雪, 等.狮子鱼皮胶原蛋白的提取及理化性质研究[J].食品工业科技, 2012, 31 (4) :65-70. [7] 段宙位, 申铉日, 陈秀明, 等.罗非鱼尾胶原蛋白的提取与鉴定[J].食品科学, 2012, 33 (6) :59-64. [8] Phanat Kittiphattanabawona, Soottawat Benjakul, Wonnop Visessanguan, et al.Isolation and Characterisation of collagen from the skin of brownbandedbambooshark (Chiloscyllium punctatum) [J].Food Chemistry, 2010, 119 (4) :1519-1526.
[9] 郭恒斌, 曾庆祝, 闫磊, 等.分光光度法测定鱼皮中羟脯氨酸含量[J].现代食品科技, 2007, 23 (7) :81-83. [10] Laemmli UK.Cleavage of structural proteins during assembly of head of bacteriophage T4[J]:Nature, 1970, 277:680-685.
[11] Elzbieta Skierka, Maria Sadowska.The influence of different acids and pepsin on extractability of collagen from the skin Baltic cod (Gadus morhua) [J].Food Chemistry, 2007, 105:1302-1306.
[12] 周爱梅, 张培丽, 刘欣, 等.淡水鱼皮胶原蛋白提取优化工艺研究 (Ⅱ) [J].食品科技, 2007, 33 (1) :113-117. [13] 林琳.鱼皮胶原蛋白的制备及胶原蛋白多肽活性的研究[D].青岛:中国海洋大学, 2006. [14] 赵苍碧, 黄玉东, 李艳辉.从牛腱中提取胶原蛋白的研究[J].哈尔滨工业大学学报, 2004, 36 (4) :516-519. [15] Lin Yung-kai, Liu Deng-cheng.Comparison of physical-chemical properties of type I collagen from different species[J].Food Chemistry, 2006, 99:244-251.
[16] 辛菲, 李华, 罗星, 等.鲅鱼皮酸溶性胶原蛋白提取及分子特性的初步研究[J].新疆农业科学, 2012, 49 (1) :122-126. [17] Doyle B B, Blout E R, Bendit EG.Infrared spectroscopy of collagen and collagen like polypeptides[J].Biopolymers, 1975, 14 (5) :937-957.
[18] Phanat Kittiphattanabawon, Soottawat Benjakul, Wonnop Visessanguan, et al.Isolation and Characterization of collagen from the skin of brownbanded bamboo shark (Chiloscyllium punctatum) [J].Food Chemistry, 2010, 119:1519-1526.
[19] Plepis A M D G, Goissis G, Das-Gupta DK.Dielectric and pyroelectric characterization of anionic and native collagen[J].Polymer Engineering and Science, 1996, 36 (24) :2932-2938.
[20] Nagai, Takeshi, Araki, et al.Collagen of the skin of ocellate puffer fish (Takifugu rubripes) [J].Food Chemistry, 2002, 78:137-177.
[21] Kimura S, OhnoY, MiyauchiY, et al.Fish skin type I collagen:wide distribution of anα3subunit in teleosts[J].Comparative Biochemistry and Physiology, 1987, 88B (1) :27-34.
[22] AOAC.Official methods of analysis (16th Ed) [M].Washington, DC:Association of Official Analytical Chemists, 1995.
[23] Nalinanon S, Benjakul s, Visessanguan W, et al.Use of pepsin for collagen extration from the skin of bigeye snapper (Priacanthus tayenus) [J].Food Chemistry, 2007, 104 (2) :593-601.
[24] 杨树奇.军曹鱼鱼皮胶原蛋白的提取及其功能特性的研究[D].广州:广东海洋大学, 2010. [25] 张建忠, 安辛欣, 王林, 等.草鱼皮酸溶性和酶溶性胶原蛋白的提取及性质[J].食品科学, 2006, 27 (12) :556-559. [26] Phanat Kittiphattanabawon, Soottawat Benjakul, Wonnop Visessanguan, et al.Isolation and Characterisation of collagen from the skin of brownbanded bamboo shark (Chiloscyllium punctatum) [J].Food Chemistry, 2010, 119:1519-1526.
[27] Muyonga J H, Cole C G B, Duodu K G.Characterisation of acid soluble collagen from skins of young and adult Nile perch (Lates niloticus) [J].Food Chemistry, 2004, 85 (1) :81-89.
[28] D Hickman, T J Sims, C A Miles, et al.Isinglass/collagen:denaturation and functionality[J].Journal of Biotechnology, 2000, 79 (3) :245-257.
[29] Sionkowska A.Thermal denaturation of UV-irradiated wet rat tail tendon collagen[J].International Journal of Biological Macromolecules, 2005, 35:145-149.
[30] 任俊莉, 付丽红, 邱化玉.胶原蛋白的应用及其发展前景[J].中国皮革, 2004, 33 (1) :36-38.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
