富锗木耳对小鼠肠道菌群的影响
详细信息Effect of germanium-riched Auricularia auricular mycelia on intestinal microflora in mice
-
摘要: 通过荧光定量PCR方法,测定了喂饲富锗木耳菌丝体小鼠肠道内大肠杆菌和乳酸杆菌的16srDNA数量,探讨了富锗木耳菌丝体对小鼠肠道菌群的影响。实验结果表明,喂饲富锗木耳菌丝体小鼠肠道内大肠杆菌数量未见明显变化(p>0.05),乳酸杆菌数量显著增加(p<0.01),富锗木耳对肠道菌大肠杆菌无明显的调节作用,对乳酸杆菌的生长繁殖有促进作用。Abstract: The numbers of 16s rDNA of Escherichia coli and Lactobacillus in the intestine of mice fed with germanium - riched Auricularia Auricular mycelia was detemined and the influence of germanium - riched Auricularia auricular mycelia on intestinal flora was investigated by fluorogenic quantitative PCR. The results showed that:no significant change of the number of Escherichia coli in the intestine of mice fed with germanium -riched Auricularia auricular mycelia was observed (p >0.05 ) , and the number of Lactobacillus significantly increased ( p < 0 . 01 ) . The germanium - riched Auricularia auricular mycelia had no significant regulation effect on Escherichia coli, but had promotive effect on Lactobacillus multiplication.
-
[1] 毕志树, 郑国扬, 李泰辉, 等.广东大型真菌志[M].广州:广东科技出版社, 1994, 8. [2] 杨月欣, 王光亚, 潘兴昌, 等.中国食物成分表[M].北京:北京大学医学出版社, 2002-12. [3] Munakata T, Arai S, Kuwano K, et al.Induction of interferon production by natural killer cells by organic germanium compound, Ge-132[J].Interferon Res, 1987, 7 (1) :69-76.
[4] 陈石良, 许正宏, 陶文沂, 等.灰树花富锗培养研究[J].无锡轻工大学学报, 2000, 19 (3) :248-251. [5] 李秋蕊.富锗羊肚菌菌丝体深层发酵的研究[D].长春:吉林大学, 2005, 6. [6] 尹计秋, 燕小梅, 牛奔.微波消解-火焰原子吸收光谱法测定海藻中的微量元素[J].光谱实验室, 2011, 28 (1) :118-120. [7] 高平章, 张英武, 肖玲玲, 等.微波消解-火焰原子吸收光谱法测定黄岑中的微量金属元素含量[J].安徽农业科学, 2010, 38 (35) :2021-2022. [8] 李云, 衷明华.微波消解-火焰原子吸收光谱法测定下火药材中的微量元素[J].光谱实验室, 27 (1) :257-259. [9] 刘瑛, 杨秀华, 卢中热.石墨炉原子吸收光谱法测定饮料中的锗及基体干扰的研究[J].分析测试学报, 1995, 14 (5) :49-52. [10] 李好.富硒猴头栽培及其药理活性初探[D].合肥:安徽农业大学, 2010, 6. [11] 杨美芬, 王玉明, 黄永坤, 等.用细菌16S rRNA荧光定量PCR法检测肠道菌群的变化[J].中国微生态学杂志, 2006, 18 (4) :266-269. [12] 白鹏, 吕玉敏, 顾芳.细菌16S rDNA荧光定量PCR法分析溃疡性结肠炎患者肠道菌群变化[J].胃肠病学和肝病学杂志, 2008, 17 (7) :566-571.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
