生防菌株洋葱伯克氏菌对草莓果实采后病害控制及安全性评价

范三红; 张婧婷; 施俊凤

doi:10.13386/j.issn1002-0306.2018.01.018

生防菌株洋葱伯克氏菌对草莓果实采后病害控制及安全性评价

1. 山西大学生命科学学院, 山西太原 030006;
2. 山西省农业科学院农产品贮藏保鲜研究所, 山西太原 030031

基金项目:

山西省重点研发项目(201703D221010-1)

山西省农业科学院博士后基金项目(BSH-2015JJ-003)。

详细信息

作者简介:
范三红(1963-),男,硕士,教授,研究方向:食品科学,E-mail:fsh729@sxu.edu.cn。

通讯作者:
施俊凤(1977-),女,博士,副研究员,研究方向:采后病害及生物防治,E-mail:sjfty@126.com。

中图分类号: TS201.3
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出版历程
- 收稿日期: 2017-05-26
- 网络出版日期: 2020-12-28
- 刊出日期: 2017-12-31

Control of strawberry fruit postharvest decay by antagonistic bacterium Burkholderia contaminans and its safety evaluation

1. College of Life Science, Shanxi University, Taiyuan 030006, China;
2. Institute of Agricultural Product Storage and Fresh Keeping, Shanxi Academy of Agricultural Science, Taiyuan 030031, China

摘要

摘要: 采用自然腐烂法和伤口接种法研究生防菌株洋葱伯克氏菌Burkholderia contaminans(简称B-1),在不同发酵时间、处理方式和处理液对草莓果实采后贮藏的影响,并进行该生防菌的大鼠急性经口毒性实验、家兔皮肤及眼刺激性实验和豚鼠皮肤变态反应(致敏)实验。结果表明:与CK相比,生防菌B-1发酵培养24 h、采后果实浸泡3 min及稀释5倍的发酵液处理草莓果实,其采后腐烂率分别下降了55.04%、46.74%和39.98%,而且发酵原液、稀释5倍发酵液及菌悬液对草莓果实伤口接种灰霉病抑制率达100%。该生防菌的大鼠急性LD₅₀>5000 mg/kg,皮肤刺激强度和眼刺激积分指数(I.A.O.I)为0,眼刺激平均指数(M.I.O.I)48 h为0,皮肤变态反应(致敏)实验致敏率为0。本研究表明该细菌作为生防菌具有安全性,为该生防菌的生产性应用奠定基础。
- 洋葱伯克氏菌 /
- 草莓 /
- 采后病害 /
- 抑制率 /
- 安全性评价
Abstract: The effects of different fermentation times, treatment methods and treatment solutions of the antagonistic bacterium Burkholderia contaminans B-1 on the postharvest storage of strawberry fruit were studied by natural decay and wound inoculation method. And the safety evaluation of the antagonistic bacterium B-1 including acute oral toxicity test on rats, skin, eyes irritation test on healthy rabbits and dermal allergy reaction on guinea pigs were performed. The results showed that inhibition rate of 24 hours culturing, 3 minutes immersing and with dilution five times of the fermented liquid were decreased by 55.04%, 46.74%, 39.98% respectively compared with the CK. The inhibition rate of gray mold on strawberry fruit reached 100% in the treatment of fermentation solution, diluted 5 times fermentation broth and bacterial suspension. The antagonistic bacteria in rats with acute LD₅₀>5000 mg/kg. The mean index of skin irritation and eye irritation(I.A.O.I)on rabbits were 0. The mean index of eye irritation(M.I.O.I)were 0 after 48 hours. Sensitization rate of skin sensitization(sensitization)test was 0. The result indicated that the bacterium is safe as a biocontrol bacterium. This study laid the foundation for the productive application of the biocontrol bacterium.
- Burkholderia contaminans /
- strawberry /
- postharvest disease /
- inhibition rate /
- safety evaluation

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参考文献(27)

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