摘要:
采用MiSeq高通量测序技术对湖北省恩施土家族苗族自治州宣恩县的3个米酒样品微生物多样性进行了解析,结果发现米酒中的细菌主要为隶属于Firmicutes(硬壁菌门)的Pediococcus(片球菌属,74.10%)、Bacillus(芽孢杆菌属,6.32%)和Lactobacillus(乳杆菌属,3.03%)以及隶属于Proteobacteria(变形菌门)的Pseudomonas(假单胞菌属,3.58%)、Acinetobacter(不动杆菌属,1.22%)和Enterobacter(肠杆菌属,1.08%),真菌主要为隶属于Ascomycota(子囊菌门)的Wickerhamomyces(异常威克汉姆酵母属,44.67%)、Saccharomycopsis(扣囊复膜酵母,23.10%)和Trichomonascus(1.20%)以及隶属于Mucoromycotina(毛霉亚门)的Amylomyces(淀粉菌,27.06%)。细菌和真菌的核心OTU包含序列条数占总序列数的89.88%和98.80%。由此可见,宣恩地区米酒存在大量的核心细菌和真菌类群。
Abstract:
Three rice wine samples were collected from Xuanen county,Enshi Tujia and Miao Autonomous Prefecture,Hubei province and MiSeq high-throughput sequencing technology were established to investigate the biodiversity of bacteria and fungi of them. The result indicated that Pediococcus,Bacillus and Lactobacillus affiliated to Firmicutes,Pseudomonas,Acinetobacter and Enterobacter affiliated to Proteobacteria were domain generas,with the relative abundance of 74.10%,6.32%,3.03%,3.58%,1.22% and 1.08%,respectively. The result also indicated that Wickerhamomyces,Saccharomycopsis and Trichomonascus affiliated to Ascomycota,Amylomyces affiliated to Mucoromycotina were domain generas,with the relative abundance of 44.67%,23.10%,1.20% and 27.06%,respectively. The sequences cluster to core bacterial and fungal OTU were 89.88% and 98.80% of total sequences. Thus,rice wine samples collected from Xuanen county shared a large number of core bacterial and fungal microbiome.
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