Ultrasonic-assisted Extraction Optimization of Total Flavonoids from Macheng Chrysanthemum morifolium and Its Antioxidant Activity
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摘要: 以麻城福白菊为原材料,采用单因素及响应面优化超声辅助提取黄酮工艺条件,并评价最优条件下黄酮提取物的抗氧化活性。结果表明,超声波功率为440 W,超声温度为60 ℃,超声时间为50 min。在此条件下福白菊总黄酮的得率为7.15%。当福白菊黄酮浓度为0.05 g/L时,总还原能力最强,此时测得吸光度值为0.341;当福白菊黄酮浓度为0.50 g/L时,福白菊黄酮对2,2-联苯基-1-苦基肼基(DPPH)自由基清除率达81.25%,其对DPPH自由基的IC50值为0.191 g/L,表明其具有较好的抗氧化活性。Abstract: With Macheng Chrysanthemum morifolium as raw material, single factor and response surface methodology were used to optimize the ultrasonic assisted extraction of flavonoids, and the antioxidant activity of total flavonoids extracts was evaluated under the optimal conditions. The results showed that the ultrasonic power was 440 W, the ultrasonic temperature was 60℃, and the ultrasonic time was 50 min. Under these optimized conditions, the total flavonoids yield was 7.15%. When the concentration of flavonoids was 0.05 g/L, the total reducing capacity was the strongest, and the absorbance value was 0.341.The free radical scavenging rate of 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) was 81.25% with 0.50 g/L flavonoids, and its IC50 for DPPH free radical was 0.191 g/L, which indicated that it had good antioxidant activity.
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