摘要:
本研究采用冷压法制备琯溪蜜柚精油,并对比分析了蜜柚精油的抗氧化性及精油抑制酪氨酸酶的效果。结果表明,在150 mg/mL时,精油对DPPH自由基清除率达到了68%,低于同浓度的BHT的清除率,半抑制浓度为70.12 mg/mL;在3 mg/mL时,精油对羟自由基清除率达到了92%,高于同浓度的VC的清除率,半抑制浓度为0.88 mg/mL;当质量浓度为1.0 mg/mL时,精油总抗氧化性能比BHT高出20.35%。而当精油质量浓度大于100 μg/mL时,酪氨酸酶激活率小于曲酸,半抑制浓度为157.45 μg/mL,具有较好的抑制B16细胞酪氨酸酶作用。由于蜜柚精油有良好的抗氧化性与美白的作用,因此在食品与化妆品等中有较好的应用前景。
关键词:
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琯溪蜜柚
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精油
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抗氧化
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酪氨酸酶
Abstract:
In this study,the oil of Guanxi pomelo oil(GP oil)was prepared by cold pressing method,and the antioxidant activity and the effect on tyrosinase of pomelo oil were compared and analyzed. The results showed that the DPPH free radical scavenging rate of GP oil reached 68% at 150 mg/mL which was lower than that of BHT,IC50 was 70.12 mg/mL. At 3 mg/mL,the hydroxyl free radical scavenging rate of GP oil was 92%,which was higher than that of VC at the same concentration,IC50 was 0.88 mg/mL;when the mass concentration was 1.0 mg/mL,the total antioxidant activity of GP oil was 20.35% higher than that of BHT. And the activation rate of tyrosinase was lower than that of kojic acid when the concentration of GP oil was more than 100 μg/mL,IC50 was 157.45 μg/mL. It means that the GP oil could inhibit the tyrosinase effect of B16 cells. As the GP oil has a good antioxidant and whitening effect,it had a better application prospects in food and cosmetics.