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中国精品科技期刊2020
王少杨,李子院,覃黎娜,等. 基于柑橘纤维的益生菌水凝胶载体制备及其控释性能研究[J]. 食品工业科技,xxxx,x(x):1−7. doi: 10.13386/j.issn1002-0306.2024040044.
引用本文: 王少杨,李子院,覃黎娜,等. 基于柑橘纤维的益生菌水凝胶载体制备及其控释性能研究[J]. 食品工业科技,xxxx,x(x):1−7. doi: 10.13386/j.issn1002-0306.2024040044.
WANG Shaoyang, LI Ziyuan, QIN Lina, et al. Preparation and Controlled Release Properties of Probiotic Hydrogel Carrier Based on Citrus Cellulose[J]. Science and Technology of Food Industry, xxxx, x(x): 1−7. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024040044.
Citation: WANG Shaoyang, LI Ziyuan, QIN Lina, et al. Preparation and Controlled Release Properties of Probiotic Hydrogel Carrier Based on Citrus Cellulose[J]. Science and Technology of Food Industry, xxxx, x(x): 1−7. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024040044.

基于柑橘纤维的益生菌水凝胶载体制备及其控释性能研究

Preparation and Controlled Release Properties of Probiotic Hydrogel Carrier Based on Citrus Cellulose

  • 摘要: 为提高益生菌在胃肠液中的存活率及释放率,本研究以柑橘纤维和羧甲基纤维素钠为包埋材料,柠檬酸为交联剂制备水凝胶。通过吸附包埋植物乳植杆菌B5,测定包埋后水凝胶的包埋率、控释性能、胆盐耐受性及储藏稳定性。结果表明,不同配比的水凝胶均具备良好的包埋效果,包埋率均高于79%;当柑橘纤维素和羧甲基纤维素钠的配比为1:4时,水凝胶的控释性能最佳,在模拟肠液中的释放率达到89.25%;载菌水凝胶能明显提高植物乳植杆菌B5在胆盐溶液中的存活率,最高达到87.09%;在4 ℃下保藏30 d后,水凝胶包埋的植物乳植杆菌B5的存活率均高于60%,当柑橘纤维素和羧甲基纤维素钠的配比为1:4时,水凝胶包埋的植物乳植杆菌B5存活率为70.66%,活菌数仍大于7 lg CFU/mL。研究表明柑橘纤维-羧甲基纤维素钠水凝胶能有效减少益生菌与外界环境中不利因素的接触,从而显著提高其在各种环境下的存活率。

     

    Abstract: To enhance the survival rate and release rate of probiotics in gastrointestinal fluids, hydrogels were prepared using citrus cellulose and sodium carboxymethylcellulose as embedding materials, with citric acid as the cross-linking agent. Lactiplantibacillus plantarum B5 was adsorbed and embedded in the hydrogels to evaluate the embedding rate, controlled release performance, bile salt tolerance, and storage stability. Results demonstrated that hydrogels with varying ratios exhibited favorable embedding effects, with an embedding rate exceeding 79%. The hydrogel with a citrus cellulose to sodium carboxymethylcellulose ratio of 1:4 showed optimal controlled release performance, achieving a release rate of 89.25% in simulated intestinal fluid. The bacteriophage-carrying hydrogel significantly enhanced the survival rate of L. plantarum B5 in bile salt solution, reaching 87.09%. The hydrogel maintained a storage stability of 30 days at 4 °C, with the survival rate of embedded L. plantarum B5 exceeding 60% after 30 days, particularly reaching 70.66% with the 1:4 ratio of citrus cellulose to sodium carboxymethylcellulose, and maintaining viable bacteria levels above 7 lg CFU/mL. Studies have shown that citrus cellulose-sodium carboxymethylcellulose hydrogels are effective in reducing the exposure of probiotics to unfavorable factors in the external environment, thus significantly improving their survival in various environments.

     

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