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中国精品科技期刊2020
代思雨,汤颖,刘念,等. 芹菜素脂质体的制备、修饰、表征及抗氧化活性研究[J]. 食品工业科技,2025,46(1):1−11. doi: 10.13386/j.issn1002-0306.2023120336.
引用本文: 代思雨,汤颖,刘念,等. 芹菜素脂质体的制备、修饰、表征及抗氧化活性研究[J]. 食品工业科技,2025,46(1):1−11. doi: 10.13386/j.issn1002-0306.2023120336.
DAI Siyu, TANG Ying, LIU Nian, et al. Preparation, Modification, Characterization and Antioxidant Activities of Apigenin Liposomes[J]. Science and Technology of Food Industry, 2025, 46(1): 1−11. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120336.
Citation: DAI Siyu, TANG Ying, LIU Nian, et al. Preparation, Modification, Characterization and Antioxidant Activities of Apigenin Liposomes[J]. Science and Technology of Food Industry, 2025, 46(1): 1−11. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120336.

芹菜素脂质体的制备、修饰、表征及抗氧化活性研究

Preparation, Modification, Characterization and Antioxidant Activities of Apigenin Liposomes

  • 摘要: 目的:为提高芹菜素水溶性、稳定性,促进其充分发挥抗氧化生物活性,本研究拟制备芹菜素脂质体(Apigenin liposomes,AP-L),并用壳聚糖(Chitosan,CS)修饰脂质体表面。方法:比较低速离心法和过膜法两种包封率测定方法的适用性。以包封率为评价指标,比较薄膜分散-超声法和乙醇注入-超声法两种AP-L制备方法,并通过单因素实验和响应面试验优化AP-L制备工艺。通过静电吸附方式将壳聚糖吸附于脂质体表面,得到壳聚糖芹菜素脂质体(Chitosan apigenin liposomes,CS-AP-L)。考察修饰前后脂质体的外观、微观形态、粒径大小及分布、电位、包封率和稳定性的变化。通过DPPH和ABTS+自由基清除实验考察芹菜素水分散体(Apigenin dispersion,AP-D)、AP-L和CS-AP-L的抗氧化能力。结果:低速离心法测定脂质体包封率更准确。薄膜分散-超声法制备AP-L包封率更高。AP-L最佳制备工艺为:芹菜素6 mg,芹菜素磷脂质量比1:24,磷脂胆固醇质量比6:1,乙醇6.3 mL,磷酸盐缓冲液20 mL,水化温度50 ℃,超声时间20 min。AP-L粒径为157.34±1.87 nm,多分散系数(Polydispersity index,PDI)为0.240±0.025,包封率为66.50%±1.00%,CS-AP-L粒径为564.22±39.7 nm,PDI为0.292±0.022,包封率为60.17%±1.97%。稳定性实验显示常温保存30 d后,AP-L渗漏率为21.92%±4.84%,CS-AP-L渗漏率为10.64%±0.28%,CS-AP-L更稳定。DPPH和ABTS+自由基清除实验表明,AP-L和CS-AP-L均可以提高芹菜素的自由基清除能力,且CS-AP-L的自由基清除能力更强。结论:本研究制备的CS-AP-L粒径大小合适,分布均匀,包封率较高、稳定性较好,具有促进芹菜素抗氧化活性的潜力,可为开发芹菜素衍生产品提供参考依据。

     

    Abstract: Objective: To improve the water solubility and stability of apigenin and promote its antioxidant biological activity, apigenin liposomes (AP-L) were developed and subsequently surface-modified with chitosan (CS). Methods: The appropriateness of two methods, low-speed centrifugation and membrane filtration, for measuring encapsulation efficiency was evaluated. Encapsulation efficiency served as the evaluation criterion to compare the AP-L preparation methods employing the film dispersion-ultrasound and ethanol injection-ultrasound techniques. Additionally, the preparation process underwent optimization via both single-factor experiments and response surface tests. Chitosan was adsorbed onto the surface of liposomes through electrostatic adsorption to produce chitosan apigenin liposomes (CS-AP-L). The appearance, microstructure, particle size and distribution, potential, encapsulation efficiency and stability of the liposomes were observed before and after chitosan modification. The antioxidant capacity of apigenin dispersion (AP-D), AP-L and CS-AP-L was investigated by DPPH and ABTS+ free radical scavenging assays. Results: The low-speed centrifugation method was found to be more precise in determining the entrapment efficiency of liposomes, while the membrane dispersion-ultrasonic method demonstrated higher encapsulation efficiency. The optimal preparation process for AP-L included 6 mg of apigenin, an apigenin to phospholipid ratio of 1:24, a phospholipid to cholesterol ratio of 6:1, 6.3 mL of ethanol, 20 mL of phosphate buffer solution (PBS), hydration temperature 50 ℃ and ultrasonic time 20 min. The particle size of AP-L was 157.34±1.87 nm, the polydispersity index (PDI) was 0.240±0.025, and the encapsulation efficiency was 66.50%±1.00%. The particle size of CS-AP-L increased to 564.22±39.7 nm, the PDI was 0.292±0.022, and the encapsulation efficiency decreased to 60.17%±1.97%. After being stored at room temperature for 30 days, the leakage rate of AP-L was 21.92%±4.84%, compared to 10.64%±0.28% for CS-AP-L. This indicated that CS-AP-L demonstrated superior stability. Furthermore, the DPPH and ABTS+ free radical scavenging experiment demonstrated that both AP-L and CS-AP-L could enhance the free radical scavenging capacity of apigenin, with CS-AP-L showing even greater scavenging capability. Conclusion: The CS-AP-L prepared in this study exhibits appropriate particle size, uniform distribution, high encapsulation efficiency, good stability, and the capability to enhance the antioxidant activities of apigenin, thereby offering valuable insights for the development of apigenin derivative products.

     

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