Abstract: In order to study the effects of GABA-rich germinated adzuki bean on blood glucose level and intestinal flora in T2DM mice. C57BL/6J mice were used as subjects to construct T2DM model by high fat diet+STZ injection. Different doses of GABA-rich germinated adzuki bean were used to treat T2DM mice for 6 weeks and 16S rRNA sequencing technique was used to identify the microbial structure and distribution in the cecal contents of T2DM mice. The results showed that different doses of GABA-rich adzuki bean dietary intervention could significantly reduce the FBG value of T2DM mice, and the FBG value of high dose GABA-rich adzuki bean (TF3) group was 8.36±0.78 mmol/L, which was 54.09%, lower than that of model group (M). In addition, TF3 diet induced significant changes in intestinal flora abundance in T2DM mice. Firmicutes abundance at gate level was 35.96%, which decreased by 53.17% compared with M model group, and Bacteroidetes and Verrucomicrobia abundance were significantly up-regulated. In summary, the improvement of glucose and lipid metabolism by TF3 diet was significantly correlated with the abundance of dominant bacteria in Bacteroidetes and Verrucomicrobia, suggesting that high dose of GABA-rich adzuki bean diet could alleviate hyperglycemia symptoms in T2DM mice through beneficial bacteria, which provided reference for further explaining the relationship between T2DM and intestinal flora.