A Hydrogel with Sustained Release of 1-MCP Affects the Fruit Quality and Disease Resistance of Postharvest Strawberry
-
Abstract: In recent years, extensive research had centered on the application of 1-methylcyclopropene (1-MCP) in fruit preservation, with a predominant focus on climacteric fruits. However, the development of efficient sustained-release products remained insufficient, and the effects of 1-MCP on the quality of non-climacteric fruits such as strawberry in postharvest were not fully understood. This research investigated the creation of a 1-MCP hydrogel-based controlled release product for strawberry preservation. The hydrogel system used in the preservative was synthesized using hydroxyethyl acrylate (HEA) and acrylic acid (AA) as monomers, with polyethylene glycol diacrylate (PEGDA) serving as a cross-linking agent. 1-MCP sustained-release hydrogel, which was referred to as 1-MCP hydrogel, was prepared by wrapping 1-MCP powder in a porous water gel system in an aqueous solvent system and initiated by diphenyl phosphorus oxide (TPO). The 1-MCP hydrogel was applied to the preservation of strawberry stored at room temperature. The results showed that the 1-MCP hydrogel had excellent 1-MCP sustained release performance, and could release 1-MCP evenly and slowly. Its release rate was 7% of the 1-MCP powder group and 3% of the 1-MCP powder solution group, respectively, at 12 h, achieving a long-term effective release. Fruit preservation experiments showed that the 1-MCP hydrogel group maintained a lower weight loss rate and decay rate, which were 20% and 21% lower than the control group on day 6, respectively. In addition, 1-MCP hydrogel maintained better fruit color, higher hardness, soluble solids content (TSS), and titratable acid content (TA). On day 2, the a* value, b* value and L* value of the experimental group were 11%, 11%, and 6% higher than the control group, respectively. The hardness, TSS, and TA on day 6 were 21%, 15%, and 18% higher than the control group, respectively. To sum up, 1-MCP hydrogel has multiple functions, such as the sustained release of 1-MCP, improving fruit quality. It is also convenient to prepare and can be used in the postharvest preservation of strawberries and potentially other fruit.摘要: 近年来，已有大量的研究探究了1-甲基环丙烯（1-methylcyclopropene，1-MCP）在水果保鲜方面的应用，这些研究大多集中于呼吸跃变型果实。然而，对于1-MCP的保鲜应用仍缺乏高效缓释产品，且其在草莓等典型非呼吸跃变果实中的作用仍待进一步探究。本研究基于水凝胶体系开发了一种用于草莓保鲜的1-MCP水凝胶控释保鲜剂。该保鲜剂的水凝胶体系利用丙烯酸羟乙酯（HEA）、丙烯酸（AA）两种单体和交联剂聚乙二醇二丙烯酸酯（PEGDA），在二苯基氧化磷（TPO）的引发下，于水溶性体系中，将1-MCP粉末包裹在多孔水凝胶体系内，制得1-MCP缓释水凝胶，并将其用于常温下草莓保鲜。结果显示，该凝胶具有优异的1-MCP缓释性能，能够均匀且缓慢地释放1-MCP气体，其第12 h的释放率分别是1-MCP粉末组和1-MCP水溶液的7%和3%，达到长期有效释放的效果。果实保鲜实验表明，该凝胶能使草莓果实实现更低的失重率、腐烂率，在第6 d时，实验组比对照组分别低了20%和21%。除此之外，1-MCP缓释水凝胶能使草莓维持更好的果实色泽、更高的硬度、可溶性固形物含量（TSS）和可滴定酸含量（TA），实验组在第2 d时，其a*值、b*值和L*值分别比对照组高了11%、11%和6%，第6 d的硬度、TSS和TA分别比对照组高了21%、15%和18%。综上，1-MCP水凝胶具有缓释1-MCP、提高果实品质等多种功能，且制备方便，可用于草莓等水果的采后保鲜。
-
关键词:
- 1-甲基环丙烯（1-MCP）水凝胶 /
- 缓释 /
- 草莓 /
- 果实品质 /
- 抗病性
-
![]()
Figure 1. Schematic diagram of 1-MCP hydrogel
Notes: AA referred to acrylic acid; HEA referred to hydroxyethyl acrylate; PEDGA referred to poly (ethylene glycol) diacrylate.
![]()
Figure 2. Release behavior of 1-MCP hydrogel, 1-MCP powder, and 1-MCP powder solution under 95% relative humidity and room temperature
Notes: Data sets marked with the asterisk are significantly different from the 1-MCP hydrogel at the same storage time (*P<0.05, **P<0.01, ***P<0.001), Fig.2 to Fig.10 are the same.
![]()
Figure 9. Effects of 1-MCP hydrogel on the total soluble solids (TSS) content of strawberry during storage
-
[1] ZHU S H, ZHOU J. Effect of nitric oxide on ethylene production in strawberry fruit during storage[J]. Food Chemistry,2007,100(4):1517−1522. doi: 10.1016/j.foodchem.2005.12.022
[2] GIAMPIERI F, ALVAREZ-SUAREZ J M, BATTINO M. Strawberry and human health:effects beyond antioxidant activity[J]. Journal of Agricultural and Food Chemistry,2014,62(18):3867−3876. doi: 10.1021/jf405455n
[3] SHARMA E, KAPOOR R. Insights into the molecular interplay of virulence factors in Botrytis cinerea[J]. Australasian Plant Pathology,2017,46(6):551−561. doi: 10.1007/s13313-017-0519-7
[4] REYMOND P, WEBER H, DAMOND M, et al. Differential gene expression in response to mechanical wounding and insect feeding in arabidopsis[J]. The Plant Cell,2000,12(5):707−720. doi: 10.1105/tpc.12.5.707
[5] HARKER F R, ELGAR H J, WATKINS C B, et al. Physical and mechanical changes in strawberry fruit after high carbon dioxide treatments[J]. Postharvest Biology and Technology,2000,19(2):139−146. doi: 10.1016/S0925-5214(00)00090-9
[6] OLIVEIRA A, ALMEIDA D P F, PINTADO M. Changes in phenolic compounds during storage of pasteurized strawberry[J]. Food and Bioprocess Technology,2014,7(6):1840−1846. doi: 10.1007/s11947-013-1239-9
[7] XU Y Q, CHARLES M T, LUO Z S, et al. Ultraviolet-C priming of strawberry leaves against subsequent Mycosphaerella fragariae infection involves the action of reactive oxygen species, plant hormones, and terpenes[J]. Plant, Cell & Environment, 2019, 42(3):815−831.
[8] FUENTES L, FIGUEROA C R, VALDENEGRO M. Recent advances in hormonal regulation and cross-talk during non-climacteric fruit development and ripening[J]. Horticulturae,2019,5(2):45. doi: 10.3390/horticulturae5020045
[9] SYMONS G M, CHUA Y J, ROSS J J, et al. Hormonal changes during non-climacteric ripening in strawberry[J]. Journal of Experimental Botany,2012,63(13):4741−4750. doi: 10.1093/jxb/ers147
[10] FIGUEROA N E, GATICA-MELÉNDEZ C, FIGUEROA C R. Ethylene application at the immature stage of Fragaria chiloensis fruit represses the anthocyanin biosynthesis with a concomitant accumulation of lignin[J]. Food Chemistry,2021,358:129913. doi: 10.1016/j.foodchem.2021.129913
[11] OPAZO M C, LIZANA R, PIMENTEL P, et al. Changes in the mRNA abundance of FcXTH1 and FcXTH2 promoted by hormonal treatments of Fragaria chiloensis fruit[J]. Postharvest Biology and Technology,2013,77:28−34. doi: 10.1016/j.postharvbio.2012.11.007
[12] PNEVSKAYA A Y, BUGAEV A L, TERESHCHENKO A A, et al. Experimental and theoretical investigation of ethylene and 1-MCP binding sites in HKUST-1 metal–organic framework[J]. The Journal of Physical Chemistry C,2021,125(40):22295−22300. doi: 10.1021/acs.jpcc.1c06503
[13] SATEKGE T K, MAGWAZA L S. Postharvest application of 1-methylcyclopropene (1-MCP) on climacteric fruits:factors affecting efficacy[J]. International Journal of Fruit Science,2022,22(1):595−607. doi: 10.1080/15538362.2022.2085231
[14] GONG Y H, SONG J, PALMER L C, et al. Tracking the development of the superficial scald disorder and effects of treatments with diphenylamine and 1-MCP using an untargeted metabolomic approach in apple fruit[J]. Food Chemistry: Molecular Sciences,2021,2:100022. doi: 10.1016/j.fochms.2021.100022
[15] KWON J G, YOO J, WIN N M, et al. Fruit quality attributes of ‘ Arisoo’ and ‘ Picnic’ apples as influenced by 1-methylcyclopropene concentration and its application frequency during cold storage[J]. Horticulturae,2021,7(11):477. doi: 10.3390/horticulturae7110477
[16] LI X R, PENG S J, YU R Y, et al. Co-application of 1-MCP and laser microporous plastic bag packaging maintains postharvest quality and extends the shelf-life of honey peach fruit[J]. Foods (Basel, Switzerland),2022,11(12):1733.
[17] CHEN M, CHEN X, RAY S, et al. Stabilization and controlled release of gaseous/volatile active compounds to improve safety and quality of fresh produce[J]. Trends in Food Science & Technology,2020,95:33−44.
[18] WU W N, NI X Y, SHAO P, et al. Novel packaging film for humidity-controlled manipulating of ethylene for shelf-life extension of Agaricus bisporus[J]. LWT,2021,145:111331. doi: 10.1016/j.lwt.2021.111331
[19] XU Y Q, CHARLES M T, LUO Z S, et al. Preharvest ultraviolet C treatment affected senescence of stored strawberry fruit with a potential role of microRNAs in the activation of the antioxidant system[J]. Journal of Agricultural and Food Chemistry,2018,66(46):12188−12197. doi: 10.1021/acs.jafc.8b04074
[20] FONTES-CANDIA C, ERBOZ E, MARTÍNEZ-ABAD A, et al. Superabsorbent food packaging bioactive cellulose-based aerogels from Arundo donax waste biomass[J]. Food Hydrocolloids,2019,96:151−160. doi: 10.1016/j.foodhyd.2019.05.011
[21] BAYLISS L E, CULLIFORD D, MONK A P, et al. The effect of patient age at intervention on risk of implant revision after total replacement of the hip or knee:A population-based cohort study[J]. Lancet (London, England),2017,389(10077):1424−1430. doi: 10.1016/S0140-6736(17)30059-4
[22] CHUNG C K, FRANSEN M F, VAN DER MAADEN K, et al. Thermosensitive hydrogels as sustained drug delivery system for CTLA-4 checkpoint blocking antibodies[J]. Journal of Controlled Release,2020,323:1−11. doi: 10.1016/j.jconrel.2020.03.050
[23] DU Y M, JIN T, ZHAO H D, et al. Synergistic inhibitory effect of 1-methylcyclopropene (1-MCP) and chlorine dioxide (ClO2) treatment on chlorophyll degradation of green pepper fruit during storage[J]. Postharvest Biology and Technology,2021,171:111363. doi: 10.1016/j.postharvbio.2020.111363
[24] BI X F, DAI Y S, ZHOU Z Y, et al. Combining natamycin and 1-methylcyclopropene with modified atmosphere packaging to evaluate plum ( Prunus salicina cv. ‘Cuihongli’) quality[J]. Postharvest Biology and Technology,2022,183:111749. doi: 10.1016/j.postharvbio.2021.111749
[25] XU F X, LIU Y F, SHAN X F, et al. Evaluation of 1-methylcyclopropene (1-MCP) treatment combined with nano-packaging on quality of pleurotus eryngii[J]. Journal of Food Science and Technology,2018,55(11):4424−4431. doi: 10.1007/s13197-018-3354-0
[26] LI G S, QIU X, MA J, et al . Research on 1-MCP detection method and release pattern based on gas chromatography[J]. Storage and Process,2021 ,21 （10 ）:101 −107 .[27] HOTCHKISS J H, WATKINS C B, SANCHEZ D G. Release of 1-methylcyclopropene from heat-pressed polymer films[J]. Journal of Food Science,2007,72(5):330−334.
[28] LI H B, XIE C, LIANG R P, et al . The Effect of low voltage electrostatic field technology (LVEF) combined with low temperature on the quality of Zhoushan yangmei during freshness preservation[J]. Science and Technology of Food Industry,2020 ,41 （7 ）:265 −270 .[29] WANG Y, ZHAI S L, YUAN Y Y, et al . Study on the antibacterial activity of lotus leaf extract and its application in strawberry preservation[J]. Agricultural Science & Technology and Equipment,2021 ,306 （6 ）:62 −64 .[30] GAO X X, JIN Y Y, ZHANG Y Y, et al . The effect of sodium octaborate on the quality of strawberry fruit[J]. Journal of Henan Institute of Science and Technology (Natural Science Edition),2022 ,50 （3 ）:1 −6 .[31] YAN J W, LUO Z S, BAN Z J, et al. The effect of the layer-by-layer (LBL) edible coating on strawberry quality and metabolites during storage[J]. Postharvest Biology and Technology,2019,147:29−38. doi: 10.1016/j.postharvbio.2018.09.002
[32] HAN-LYN F, MARYAM-ADILAH Z A, NOR-KHAIZURA M A R, et al. Application of modified atmosphere and active packaging for oyster mushroom ( Pleurotus ostreatus)[J]. Food Packaging and Shelf Life,2020,23:100451. doi: 10.1016/j.fpsl.2019.100451
[33] ARIYANTO H D, CHIBA M, OGUMA K, et al. Release behavior of 1‐methylcylopropene coated paper‐based shellac solution in response to stepwise humidity changes to develop novel functional packaging for fruit[J]. Packaging Technology and Science,2019,32(10):523−533. doi: 10.1002/pts.2468
[34] CHOI S T, DONALD J H. Differential sorption of 1-methylcyclopropene to fruit and vegetable tissues, storage and cell wall polysaccharides, oils, and lignins[J]. Postharvest Biology and Technology,2009,52:62−70. doi: 10.1016/j.postharvbio.2008.11.002
[35] FU L, LIU S Y, XU F X . The effect of different concentrations of 1-MCP on the shelf life quality of strawberry fruits[J]. Journal of Liaoning University (Natural Science Edition),2015 ,42 （3 ）:280 −284 .[36] BLANPIED G D. A study of ethylene in apple, red raspberry, and cherry[J]. Plant Physiology,1972,49(4):627−630. doi: 10.1104/pp.49.4.627
[37] CHEN J X, MAO L C, MI H B, et al. Involvement of abscisic acid in postharvest water-deficit stress associated with the accumulation of anthocyanins in strawberry fruit[J]. Postharvest Biology and Technology,2016,111:99−105. doi: 10.1016/j.postharvbio.2015.08.003
-
期刊类型引用(6)
1. 张琪婧，耿振甲，李敏，王俊秋，钱思颖，师聪. 覆盆子总黄酮的酶辅助闪式提取及其抗氧化、降血糖和降血脂活性分析. 粮食与油脂. 2025(01): 115-120+126 . 
百度学术
2. 田良良，殷志如，曹光昭，夏裕发，张晶晶，杨洪军. 基于代谢组学和生物信息学的西洋参三七丹参颗粒抗衰老作用及分子机制研究. 中国中药杂志. 2024(07): 1932-1946 . 百度学术
3. 况慧云，孙萍东，林金元，杨立年，王慧，戴惠学，胡颖雄，郑洪建. 玉米须多糖的理化性质和体外生物活性研究. 上海农业学报. 2024(02): 24-30 . 百度学术
4. 黎楚楚，徐霖，林长松. 响应面优化黄姜花根多糖提取工艺及其抗氧化活性分析. 饲料研究. 2024(11): 109-113 . 百度学术
5. 苗琳，曲秋颖，段媛媛，衣少鹏，徐钰菲，王坤宁，宋政建. 西洋参多糖分离纯化及其生物活性研究进展. 中国果菜. 2024(10): 31-35 . 百度学术
6. 徐清华，王国明，谢丽娟，宋莹莹，郭畅冰，冯惠柳，李健豪，于晶，徐芳菲. 西洋参花多糖的提取工艺优化及其含量测定. 数理医药学杂志. 2024(11): 806-814 . 百度学术
其他类型引用(2)
下载:
计量
- 文章访问数: 486
- HTML全文浏览量: 20
- PDF下载量: 29
- 被引次数: 8
下载:
