Abstract: In this paper, the drying efficiency of natural drying, hot air and vacuum microwave on Auricularia auricula were systematically investigated, and the changes of physicochemical properties of Auricularia auricula and the degradation effect of quinolones (enrofloxacin, ciprofloxacin) were also evaluated. The results showed that the drying time of Auricularia auricula was greatly shortened to 150 min with the hot air drying (80 ℃) and 15 min with vacuum microwave (2.4 kW) compared with the natural drying. With the treatments of hot air drying and vacuum microwave drying, there was no significant effect on the content of crude protein, fat, fiber and ash in Auricularia auricula, and the total sugar content decreased by 1.34% and 2.28%, respectively. The physicochemical indexes still met the National Standard requirements. In addition, the degradation rates of enrofloxacin and ciprofloxacin during natural drying were 16.32% and 14.22%, while that of hot air drying were 35.37% and 32.39%, and vacuum microwave drying were 36.11% and 33.19%, respectively. The results indicated that there was no significant difference in antibiotic degradation between the two treatments. Therefore, vacuum microwave drying exhibits the advantages of less treatment time and effect on the physicochemical indexes, higher drying efficiency and better antibiotic degradation effect, which provides a new idea for the drying and quality control of edible fungi.