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中国精品科技期刊2020
侯润庚,丁骁,杨力颖,等. 西印度醋栗枝叶提取物的α-葡萄糖苷酶抑制活性及安全性评价[J]. 食品工业科技,2023,44(7):252−259. doi: 10.13386/j.issn1002-0306.2022070054.
引用本文: 侯润庚,丁骁,杨力颖,等. 西印度醋栗枝叶提取物的α-葡萄糖苷酶抑制活性及安全性评价[J]. 食品工业科技,2023,44(7):252−259. doi: 10.13386/j.issn1002-0306.2022070054.
HOU Rungeng, DING Xiao, YANG Liying, et al. α-Glucosidase Inhibitory Activity and Safety Evaluation of Branch and Leaf Extracts of Phyllanthus acidus[J]. Science and Technology of Food Industry, 2023, 44(7): 252−259. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070054.
Citation: HOU Rungeng, DING Xiao, YANG Liying, et al. α-Glucosidase Inhibitory Activity and Safety Evaluation of Branch and Leaf Extracts of Phyllanthus acidus[J]. Science and Technology of Food Industry, 2023, 44(7): 252−259. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070054.

西印度醋栗枝叶提取物的α-葡萄糖苷酶抑制活性及安全性评价

α-Glucosidase Inhibitory Activity and Safety Evaluation of Branch and Leaf Extracts of Phyllanthus acidus

  • 摘要: 对西印度醋栗(Phyllanthus acidus Skeels)枝叶提取物的α-葡萄糖苷酶抑制活性及安全性进行评价,为该植物的安全食用和药用研发提供参考。采用溶剂回流提取得到西印度醋栗枝叶的全组分样品,然后按照极性大小进一步对全组分进行分组划段,分别得到石油醚、乙酸乙酯和正丁醇组分,以及乙醇洗脱组分和水溶出组分;以半抑制浓度(IC50)为评价指标,比较不同组分的α-葡萄糖苷酶抑制活性。通过经典急毒实验法测定各组分对小鼠的近似最大耐受量(MTD)和半数致死量(LD50),将全组分样品进行连续灌胃20 d评价其亚急性毒性。结果表明,西印度醋栗枝叶各组分对α-葡萄糖苷酶均有一定的抑制能力;全组分的MTD为2.0 g/kg,不同组分样品的毒性大小为乙醇洗脱组分>水溶出组分>石油醚组分>乙酸乙酯组分>正丁醇组分>全组分。主要急性毒性症状是惊跳随后怠动、扭体、呼吸急促等。亚急性毒性试验未见明显毒性反应及病变,表明在口服摄入西印度醋栗全组分2 g/kg/d(相当于干燥枝叶8.6 g/kg)是安全的,具有开发为降血糖、减肥食品和药品的潜力。

     

    Abstract: To provide reference for the safety consumption of Phyllanthus acidus (PA) as food and its further medicinal development and utilization, a comparative study on acute and short-termtoxicity and α-glucosidase inhibitory ability of different components of its branches and leaves was conducted. The crude extract of PA, prepared under aqueous ethanol reflux extraction, was partitioned successively with petroleum ether, ethyl acetate and N-butanol according to the polarity. Furthermore, an alcohol-eluted and water-eluted fractions were obtained from water-soluble residue by column chromatography. The half inhibitory concentration (IC50) of α-glucosidase was used as the evaluation index to compare the in vitro hypoglycemic activity of different extracts. The acute toxicity of these fractions was screened by maximum tolerated dose (MTD), median lethal dose (LD50) values, and their subacute toxicity was evaluated by continuous oral administration of samples for 20 days. The results showed that each component of PA had a certain inhibitory ability to α-glucosidase. The MTD of crude extract of PA was 2.0 g/kg, and the order of acute toxicity for different fractions was ethanol-eluted fraction>water-eluted fraction>petroleum ether fraction>ethyl acetate fraction>N-butanol fraction>crude extract. The main acute toxicity symptoms were startle followed by idling, writhing, and shortness of breath. Subacute toxicity test showed no obvious toxicity and lesions. It was indicated that it was safe to take 2 g/kg/d (equivalent to 8.6 g/kg of dried branches and leaves) of the whole component of PA at oral ingestion. Thus, they have the potential to be developed as hypoglycemic, weight-loss food and medicine.

     

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