Abstract: Aiming at the problem of benzo(α)pyrene residues in linseed oil, a physical adsorption method was used to remove benzo(α)pyrene from linseed oil. The main research methods were as follows: Activated carbon and activated clay were used as adsorbents, and the adsorption rates of the three adsorbents to benzo(α)pyrene were compared by using the two alone or in combination. The content of unsaturated fatty acids in linseed oil before and after treatment by the removal device was identified and analyzed by quadrupole-time-of-flight tandem mass spectrometry combined with NIST standard library. Through the determination of the removal capacity of the three adsorbents, the results showed that when the fixed dosage of activated clay was 4%, benzo(α)pyrene removal rate was nearly 40%; When the amount of activated carbon was 2%, the removal rate reached 83%; When the two were used in series, the removal rate could reach 96%. In addition, through the determination of the removal capacity of three adsorbents, the results showed when the concentration of benzo(α)pyrene was less than 15 μg/kg, activated clay was used for adsorption and filtration; When the concentration of benzo(α)pyrene was 15~30 μg/kg, activated carbon adsorption filtration was chosen; When the concentration of benzo(α)pyrene was more than 30 μg/kg, used the two in series for adsorption and filtration. Among them, the best effect of removing benzo(α)pyrene from linseed oil was activated clay and activated carbon in series by physical adsorption. The optimum conditions were as follows: The ratio of activated clay and activated carbon in series was 0.8%+4%, and the removal rate could reach 96% under these conditions. The use of adsorbents did not cause loss of unsaturated fatty acids in linseed oil. This paper provides a theoretical basis for removing of benzo(α)pyrene in flaxseed oil.