Abstract: Objective: To explore the effect of yam gruel on intestinal permeability in T2DM rats. Methods: 35 male Wistar rats were randomly divided into blank group (n=8) and model group (n=27). The rats in the blank group were given normal diet, and the model group were fed with high-fat diet and intraperitoneal injection of streptozotocin to prepare T2DM rat model. After modeling, the rats were randomly divided into model group, yam gruel group and metformin group. The blank group and model group were given 5 mL normal saline, the yam gruel group was given 5 mL yam gruel, and the metformin group was given 5 mL metformin. Fasting blood glucose (FBG) was measured every week. After the intervention, the histological morphology of colon was observed, and the protein expressions of TNF-α, IL-6, Occludin and ZO-1 in colon tissue, and the expression of Zonulin, LPS, TNF-α, IL-6 and fins in serum were detected. HOMA-IR was calculated. Results: In the model group, the intestinal villi were incomplete and sparsely spaced, accompanied by neutrophil exudation. The above situation in yam gruel group and metformin group was better than that in the model group. In the model group, the protein expression of TNF-α and IL-6 increased (P<0.05), the protein expression of Occludin and ZO-1 decreased (P<0.05), the expression of Zonulin, LPS, TNF-α, IL-6 and FINS increased (P<0.05), FBG and HOMA-IR increased (P<0.05). In the yam gruel group, the protein expression of TNF-α and IL-6 decreased (P<0.05), the protein expression of Occludin and ZO-1 increased (P<0.05), the expression of Zonulin, LPS, TNF-α, IL-6 and FINS decreased (P<0.05), FBG and HOMA-IR decreased (P<0.05). Conclusion: Yam gruel could reduce the intestinal permeability of T2DM rats, and then reduce the systemic inflammatory response, alleviate insulin resistance and improve blood glucose.