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中国精品科技期刊2020
徐诺,姚哲渊,车金水,等. 离子色谱-积分脉冲安培法检测黄酒中的阿拉伯糖、半乳糖、甘露糖、葡萄糖、核糖、乳糖[J]. 食品工业科技,2022,43(3):254−259. doi: 10.13386/j.issn1002-0306.2021050209.
引用本文: 徐诺,姚哲渊,车金水,等. 离子色谱-积分脉冲安培法检测黄酒中的阿拉伯糖、半乳糖、甘露糖、葡萄糖、核糖、乳糖[J]. 食品工业科技,2022,43(3):254−259. doi: 10.13386/j.issn1002-0306.2021050209.
XU Nuo, YAO Zheyuan, CHE Jinshui, et al. Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric Detection[J]. Science and Technology of Food Industry, 2022, 43(3): 254−259. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021050209.
Citation: XU Nuo, YAO Zheyuan, CHE Jinshui, et al. Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric Detection[J]. Science and Technology of Food Industry, 2022, 43(3): 254−259. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021050209.

离子色谱-积分脉冲安培法检测黄酒中的阿拉伯糖、半乳糖、甘露糖、葡萄糖、核糖、乳糖

Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric Detection

  • 摘要: 目的:以保留时间定性、外标法定量,建立离子色谱-积分脉冲安培法同时检测黄酒中的阿拉伯糖、半乳糖、甘露糖、葡萄糖、核糖、乳糖,并对这几种糖的含量进行探讨。方法:色谱分离选用CarboPacTM 10(250 mm×4 mm)分析柱,以氢氧化钠和无水乙酸钠为淋洗液进行梯度洗脱,流速为 1.0 mL·min−1,柱温为30 ℃的色谱条件,在20 min内实现6种糖的分离,利用建立的方法对26个黄酒样品中的单糖含量进行测定。结果:该方法的重现性(RSD)≤3.70%,决定系数R2≥0.9990,加标回收率为91.6%~109.1%,最低检出限为2.99×10−3~1.38×10−2 µg·mL−1,表明了该方法灵敏度高、准确性好、精密度高。结论:黄酒中主要存在的单糖是葡萄糖,含量为14.31~60.42 g/L,阿拉伯糖、半乳糖、甘露糖、核糖和乳糖的含量较低;半甜型黄酒中葡萄糖的含量高于加饭酒,其含量的差异可能与酿造工艺有关。

     

    Abstract: Objective: To establish an ion chromatography-integral pulsed amperometric detection for simultaneous determination of arabinose, galactose, mannose, glucose, ribose and lactose in Chinese yellow rice wine, and to discuss the contents of these sugars. Methods: The chromatographic separation was carried out on a CarbopActM10 (250 mm×4 mm) column with sodium hydroxide and anhydrate sodium acetate as eluents. The flow rate was 1.0 mL·min−1, and the column temperature was 30 ℃. The separation of six sugars was achieved within 20 min and the established method was used to determine the monosaccharide content in 26 yellow rice wine samples. Results: The reproducibility (RSD) of the method was less than 3.70%, the determination coefficient R2 was ≥0.9990, the recoveries were 91.6%~109.1%, and the lowest detection limit was 2.99×10−3~1.38×10−3 µg·mL−1. Conclusions: The main monosaccharide in yellow rice wine was glucose, and the contents of arabinose, galactose, mannose, ribose and lactose were low. The content of monosaccharide in semi-sweet yellow rice wine was higher than that in rice wine, and the difference was mainly related to brewing technology.

     

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