Abstract: With Agriophyllum sargassum powder, mixed grain powder, mixed beans powder and filling powder as the main raw material, the D-optimal mixture design was used to study the effect of different mix proportion on the comprehensive score of simulated Agriophyllum sargassum compound meal replacement powder, the regression model was established between comprehensive score with distribution proportion, the interaction effect of each raw material in the formulations was researched, the optimal formulations of Agriophyllum sargassum compound meal replacement powder was determined as follows: Agriophyllum sargassum powder 22%, mixed grain powder 39%, mixed beans powder 35% and filling powder 4%. Under this formula condition, the protein nutrition value analysis of meal replacement powder showed that the SRCAA and EAAI values of meal replacement powder were 82.02 and 63.14, respectively, indicating that the protein nutrition of meal replacement powder was better. Combined with BV, NI, SRCAA and EAAI indexes, the nutritional value of meal replacement meal protein was comprehensively evaluated. The results showed that the Agriophyllum sargassum compound meal replacement powder had higher nutritional value.