Abstract: Objective: To study the uric acid-lowering and renal protective effects of Portulaca oleracea to provide reference for clinical research. Methods: Male ICR mice were randomly divided into 5 groups. Blank group (CON), model group (HUA), allopurinol positive drug control group (ALLOP, 0.02 g/kg/d), purslane high-dose group (PO-H, 1.48 g/kg/d), Portulaca low-dose group (PO-L, 0.5 g/kg/d). Each group was given the corresponding dose for 21 d continuously, starting from the 7th day, except for the blank group, each group was intraperitoneally injected with hypoxanthine (HX, 0.3 g/kg) combined with potassium oxazine (OAPS, 0.3 g/kg) to establish a hyperuricemia model. Observe serum uric acid (SUA) and malondialdehyde (MDA) levels in mice, collect liver to detect xanthine oxidase (XOD) and superoxide dismutase (SOD) levels, collect kidney to detect glutathione (GSH), glutathione peroxidase (GSH-PX) level. 24 hours urine was collected the day before the end of the experiment to detect urine uric acid (UUA) and urine creatinine (UCr) levels, pathological observation of mouse kidney HE staining was performed. Results: Compared with the CON group, the HUA group significantly (P<0.01) increased the levels of MDA, SUA, and XOD, and significantly (P<0.01) decreased the levels of SOD, GSH, GSH-PX, UUA, and UCr, indicating that hyperuricemia was a cause of hyperuricemia. The model was successful. Compared with HUA group, PO-H group could significantly (P<0.01) reduce SUA, MDA, XOD (levels, significantly (P<0.01), increase SOD, GSH, GSH-PX, UCr levels. PO-L group could significantly (P<0.05 or P<0.01) reduce the levels of SUA, MDA, XOD, and significantly (P<0.05 or P<0.01) increase the levels of GSH, GSH-PX, UCr. HE staining results showed that PO could significantly improve the kidneys of HUA mice tissue pathological changes. Conclusion: Portulaca oleracea extract would have certain uric acid-lowering and renal protective functions on hyperuricemia mice.