QuEChERS-UPLC-MS/MS法测定鸡蛋中19种氨基甲酸酯类农药及其代谢物残留量

Determination of 19 Carbamate Pesticide and Metabolites Residues in Eggs by QuEChERS-UPLC-MS/MS

摘要: 采用QuEChERS-超高效液相色谱-串联质谱法（UPLC-MS/MS）同时测定鸡蛋中的19种氨基甲酸酯类农药及其代谢物残留量。鸡蛋样品通过乙腈提取，分散固相萃取净化，UPLC-MS/MS测定。19种氨基甲酸酯类农药及其代谢物残留在0.2~50 ng/mL范围内线性良好（R²>0.999），方法的检出限为1.0~10 µg/kg。在添加水平为10、20、50 µg/kg时，回收率为69.3%~107.6%，相对标准偏差低于9.21%。该方法简单快速，高效准确，回收率高，能够满足鸡蛋中19种氨基甲酸酯类农药及其代谢物残留检测与确证的需要。

Abstract: The residues of 19 carbamate pesticides and their metabolites in eggs were determined by QuEChERS-UPLC-MS/MS. Egg samples were extracted by acetonitrile, purified by dispersion solid phase extraction, and determined by UPLC-MS/MS. The linearity of 19 carbamate pesticides and their metabolites in the range of 0.2~50 ng/mL was good (R²>0.999), and the detection limit of the method was 1.0~10 μg/kg. The recoveries were 69.3%~107.6 % and RSD was lower than 9.21% at 10, 20 and 50 μg/kg. The method is simple, rapid, efficient and accurate with high recovery, and can meet the needs of detection and confirmation of 19 carbamate pesticides and their metabolites residues in eggs.