Abstract: Electrospinning technology was used to prepare peanut protein isolate (PPI)-polylactic acid (PLLA) composite nanofiber film loaded with tea polyphenols (TP), and to study its microscopic morphology, infrared spectrum structure, thermal stability, hydrophobicity, and crystals structure and antibacterial properties, etc. Fourier infrared spectroscopy and X-ray diffraction results showed that the composite nanofibers had successfully embedded TP. The experimentally added TP had little effect on the morphological distribution of PPI/PLLA nanofibers. The average diameter increased from (185±57) nm to (222±72) nm. The hydrophobicity and thermal stability were reduced, but the TP/PPI/PLLA composite nanofiber membrane improved the thermal stability of TP. The antibacterial results showed that the width of the inhibition zone of TP/PPI/PLLA ternary composite nanofiber membrane against Escherichia coli and Staphylococcus aureus increased from (0.75±0.03) and (1.49±0.08) cm to (1.10±0.02) and (1.82±0.10) cm, respectively. And the antibacterial effect of Staphylococcus aureus was greater than that of Escherichia coli. This study shows that the TP/PPI/PLLA ternary composite nanofiber membrane has good potential application value.