Abstract: In order to clarify the effects of different gas microenvironments on the quality and volatile components of blueberry during controlled freezing point storage (−0.5±0.3 ℃), the changes of quality and volatile components of blueberries during 0~80 days under different gas microenvironments were studied using ‘Legacy’ blueberries as samples. The results showed that during the storage period, in the three different atmosphere control steps, the O₂ variation range was 15.4%~18.2%, 13.1%~15.9% and 6.1%~13.4%, and the CO₂ variation range was 2.2%~4.5%, 4.5%~8.8% and 8.8%~14.8%. Compared with the control, the microenvironmental gas regulation could delay the decrease of nutrient substances (V_C, anthocyanins and titratable acid) and hardness of blueberry fruits, maintained a low decay rate and a high fruit frost coverage index, and could also inhibit the production of aldehydes and promote the production of terpenoids. Among them, the gas microenvironment with O₂ content maintained at 6.1%~13.4% and CO₂ content maintained at 8.8%~14.8% has the best effect on blueberry storage, which was conducive to the long-term storage of blueberry fruits.