Abstract:
In order to explore the effect of high sucrose diet on the development, life span and antioxidant capacity of
D. melanogaster, life span, locomotion capacity and antioxidant ability of
D. melanogaster were measured through feeding high sucrose-diets to
D. melanogaster. At the same time, transcriptome sequencing of high sucrose
D. melanogaster was performed to find out the mechanism that high sucrose affected the development of
D. melanogaster at the gene transcription level. The results showed that compared with the control group, the average life span of female flies, male flies and mixed flies was shortened by 35.01%、43.01% and 34.15%, respectively in the high-sucrose group. The survival curve of male
D. melanogaster and mixed
D. melanogaster were significantly shortened than that of the control group（
P<0.05）. Compared with the control group, high sucrose diet reduced pupation rate, eclosion rate and climbing ability of flies. In the high-sucrose group, T-SOD activities and CAT activities were decreased in male
D. melanogaster, while MDA content was increased. In addition, through transcriptome sequencing, we found that the differentially expressed genes of the high-sugar group flies were enriched in the development process, the regulation of metabolic activities, and the stress response of flies. This study showed that high sucrose diet could significantly reduce the life span, development, locomotion capacity and antioxidant capacity of
D. melanogaster, it would provide reference for healthy diet.
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