Abstract: Four strains of lactic acid bacteria peptidoglycan (PG) are used as biosorbent to study the adsorption characteristics of acrylamide (AA). The effects of different factors ( pH value, temperature, time, PG concentration, AA concentration and calcium ion concentration) and simulated gastrointestinal environment on the adsorption characteristics of AA were investigated by HPLC. The results showed that with the increase of pH value and temperature, the adsorption rate of four lactic acid bacteria PG for AA increased first and then decreased. When the pH value was 5 and the temperature was 37 ℃, the adsorption rate of PG by four lactic acid bacteria reached the maximum value, among which the PG adsorption rate of Lactobacillus plantarum 1.0665 was 87.35%. Within 6 h, the adsorption rate of PG by four strains of lactic acid bacteria increased significantly with the extension of time (P<0.05), but there was no change after 6 h .The results showed that the adsorption rate decreased with the increase of AA concentration, and increased with the increase of PG concentration and calcium concentration. In the simulated gastric environment, different pH values significantly affected the adsorption effect of PG on AA (P<0.05), and the adsorption rate of PG to AA was the highest when pH was 3.5, and the different time had no significant effect on the adsorption capacity of PG for AA (P>0.05). In the simulated intestinal environment, bile salt concentration and the adsorption time significantly affected by efficiency of PG to AA (P<0.05), and 0.3%~0.4% bile salt concentration was more conducive to the adsorption of AA by PG. In conclusion, this study laid a theoretical foundation for the study of the adsorption mechanism of lactic acid bacteria.