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中国精品科技期刊2020

Safety Assessment of Two New Lactobacillus Plantarum Strains Isolated from Traditional Fermented Food of Northeast China

Victoria Yosea Mliga, YANG Liu, MA He, SUN Chang, Yohana James Mgale, REN Dayong

Victoria Yosea Mliga, YANG Liu, MA He, et al. Safety Assessment of Two New Lactobacillus Plantarum Strains Isolated from Traditional Fermented Food of Northeast China[J]. Science and Technology of Food Industry, 2021, 42(13): 253−261. (in English with Chinese abstrac). doi: 10.13386/j.issn1002-0306.2020100086.
Citation: Victoria Yosea Mliga, YANG Liu, MA He, et al. Safety Assessment of Two New Lactobacillus Plantarum Strains Isolated from Traditional Fermented Food of Northeast China[J]. Science and Technology of Food Industry, 2021, 42(13): 253−261. (in English with Chinese abstrac). doi: 10.13386/j.issn1002-0306.2020100086.
Victoria Yosea Mliga,杨柳,马赫,等. 东北地区传统发酵食品中两株植物乳杆菌的安全性评价[J]. 食品工业科技,2021,42(13):253−261. doi: 10.13386/j.issn1002-0306.2020100086.
引用本文: Victoria Yosea Mliga,杨柳,马赫,等. 东北地区传统发酵食品中两株植物乳杆菌的安全性评价[J]. 食品工业科技,2021,42(13):253−261. doi: 10.13386/j.issn1002-0306.2020100086.

Safety Assessment of Two New Lactobacillus Plantarum Strains Isolated from Traditional Fermented Food of Northeast China

More Information
    Author Bio:

    Victoria Yosea Mliga (1994−), 女,硕士研究生,研究方向:食品安全与控制,E-mail:vmliga6@gmail.com

    YANG Liu: 杨柳 (1996−), 女,硕士研究生,研究方向:农产品加工,E-mail:yl810209405@163.com

    Corresponding author:

    REN Dayong: 任大勇(1979−) ,男,博士,副教授,研究方向: 食品微生物,E-mail: rdy79@163.com

东北地区传统发酵食品中两株植物乳杆菌的安全性评价

详细信息
  • 中图分类号: TS201.3

  • Abstract: This study aimed to evaluate the safety of previously isolated Lactobacillus strains (L. Plantarum L12 and L20) by in vitro and in vivo methods. Antibiotic sensitivity, bile salt hydrolysis activity, D-lactic acid content, and hemolytic activity were analyzed in vitro. Then, a short-term oral toxicity study was conducted in mice, which received 0.2 mL (109 CFU/mL) of experimental bacterial solutions orally for 30 days. The results showed that both strains had good antioxidant activity, high sensitivity to antibiotics, high bile salt hydrolase activity, low D-lactic acid content, and gamma hemolysis. Intragastric administration had no adverse effects on growth, behavior, food intake, organ weight, or histopathological analysis of the treated mice. Compared with control mice, the serum malondialdehyde concentration and liver glutathione content of probiotic-treated mice were statistically different (P<0.05). These results suggest that strains L12 and L20 are nonpathogenic and likely to be safe for human consumption.
    摘要: 针对已分离的植物乳杆菌L12和L20通过体外和体内两种方法进行安全性评价。通过体外实验测定乳酸水解活性抗生素敏感性,胆盐水解酶活性,D-乳酸含量,γ溶血等指标;体内实验是对小鼠进行短期口服毒性的研究,口服0.2 mL(109 CFU/mL)的实验性细菌溶液30 d。结果表明,体外实验中,两株菌株均具有良好的抗氧化活性,对抗生素敏感性高,胆盐水解酶活性高,D-乳酸含量低,γ溶血;体内实验中灌胃给药对治疗小鼠的生长、行为、摄食量、器官重量或组织病理学分析均无不良影响。与对照组相比,益生菌组小鼠血清MDA浓度和肝脏GSH含量差异有统计学意义(P<0.05)。这些结果表明,菌株L12和L20是非致病性的,人直接食用是安全的。
  • Figure  1.   Hemolytic activity in treated and control groups

    Figure  2.   Weight of conventional mice

    Figure  3.   Photomicrographs of liver paraffin section of mice (a) control (b) L12, and (c) L20

    Figure  4.   Serum MDA concentration (a) and total Liver GSH concentration (b) in control and probiotic-treated mice

    Note: Values are means with standard deviations shown by vertical bars (n=4). Bars with a different lowercase letter (s) in the same treatments indicate significant differences at P<0.05 among the treatments.

    Table  1   Antibiotic susceptibility of Lactobacillus strains L12 and L20

    AntibioticL12L20
    Diameters (mm)SensitivityDiameters (mm)Sensitivity
    Rifampin22±0.2gS31±0.3bS
    Vancomycin15±0.1jI8.3±0.03jR
    Kanamycin7±0.5llR27±0.4dS
    Ceftazidime30±0.1cS14±0.05iI
    Sulphamethoxazole/Trimethoprim30±0.02cS17±0.2hI
    Erythromycin32±0.04bS27±0.03dS
    Ampicillin24±0.07fS26±0.05eS
    Penicillin G33±0.0aS31±0.2bS
    Streptomycin26±0.03eS21±0.1gS
    Gentamicin29±0.04dS29±0.03cS
    Tetracycline16±0.04iI32±0.1aS
    Cefotaxime2.9±0.05mR23±0.2fS
    Ciprofloxacin20±0.2hS2.3±0.03kR
    Norfloxacin14±0.01kS1.6±0.6lR
    Note: The different lowercase letters in the table indicate a significant difference (P<0.05);R=is low -sensitive or resistant, S=is highly sensitive, I=is moderately sensitive.
    下载: 导出CSV

    Table  2   Test results of D-lactic acid content and bile salt hydrolase activity of strains

    StrainBile salt hydrolase activityD-lactic acid content
    Absorbance valuesCholecystolytic enzyme (μmol/L)Absorbance valuesD-lactic acid concentration (μmol/L)
    L1243.51±0.25a227.32±1.89b4.99±0.25a18.87±15.36b
    L2059.78±0.38b245.92±2.0a6.47±0.38a21.53±20.25a
    Control0.11±0.01b0.62±2.20c
    Note: The different lowercase letters in the table indicate a significant difference (P<0.05).
    下载: 导出CSV

    Table  3   Treatment-related sign of toxicity during the 30-days study period

    Organ systemObserved & inspected itemsPerformance after gavage
    Central nervous systemBehaviourNo abnormal calls, no abnormal posture and repeated scratching
    Neuromuscular systemActionNormal movement, no movement disorder
    Autonomic nervous systemPupil sizeNormal, no reduction or expansion
    SecretionNo salivation, no tears
    Respiratory systemNostrilsNo flow
    Respiration rateNormal breathing
    Digestive systemBelly typeAbdominal type is normal
    Fecal shape and colorStool formation, dark yellow
    Urogenital systemPerineumNo secretions
    Mammary glandNo swelling
    Skin and coatColor, tensionNormal, no sagging, no rash
    CompletenessNo hair removal, no erect
    EyeEyelidNo upper eyelid droop
    EyeballNo protrusion, no corneal opacity, no congestion of the conjunctiva
    TransparencyNot turbid
    下载: 导出CSV

    Table  4   Organ relative weights of control and probiotic-treated mice

    StrainConcentration (CFU/mL)Gavage dose (mL)Visceral index (organ-body ratio)
    SpleenHeartLiverKidney
    L121090.20.008±0.000280.008±0.000050.05±0.00220.06±0.001
    L201090.20.009±0.00030.008±0.000250.06±0.0040.06±0.003
    Control1090.20.009±0.00050.008±0.000220.06±0.0030.06±0.0013
    Note: There is no significant difference between the experimental group and the control group (P>0.05).
    下载: 导出CSV
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出版历程
  • 收稿日期:  2020-10-13
  • 网络出版日期:  2021-04-21
  • 刊出日期:  2021-06-30

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