小麦肽的抗氧化与抗疲劳作用的研究

王倩倩; 杜鹃; 陈鸣; 冯凤琴

doi:10.13386/j.issn1002-0306.2020100066

摘要: 目的：探讨小麦肽的体外抗氧化活性和体内抗疲劳作用。方法：通过H₂O₂诱导小鼠成纤维细胞L929构建氧化应激损伤模型，测定损伤后L929细胞的存活率、乳酸脱氢酶（lactic dehydrogenase, LDH）、超氧化物歧化酶（superoxide dismutase, SOD）和谷胱甘肽过氧化物酶（glutathione peroxide dismutase, GSH-Px）活力以及丙二醛（MDA）含量，从细胞水平评价小麦肽的抗氧化作用。然后通过力竭游泳和自由泳实验，测定力竭游泳时间、乳酸（lactic acid, LA）、尿素氮（blood urea nitrogen, BUN）和肌糖原（muscle glycogen, MG）含量、SOD和GSH-Px活力以及MDA含量，来评价小麦肽的体内抗疲劳和抗氧化作用。结果：小麦肽浓度在0.4~0.8 mg/mL时对L929细胞的H₂O₂损伤有极显著的保护作用（P<0.01)；与模型组相比，小麦肽浓度为0.6和0.8 mg/mL时的LDH活力分别显著下降了20.79%和19.67%（P<0.05)，SOD活力分别显著（P<0.05)和极显著（P<0.01)提高了83.21%和95.19%，GSH-Px活力分别提高了28.69%和32.14%，MDA含量分别显著下降了25.91%和26.99%（P<0.01) 。体内抗疲劳实验表明，与对照组相比，2个剂量组的小鼠力竭游泳时间分别极显著延长了72.93%和91.73%（P<0.01)，LA含量分别极显著下降了24.65%和25.16%（P<0.01)，BUN含量分别极显著（P<0.01)和显著（P<0.05)下降了19.74%和17.78%，MG含量分别极显著提高了48.63%和56.85%（P<0.01)；体内抗氧化结果表明，2个剂量组的SOD和 GSH-Px活力分别极显著提高了20.54%、25.91%和29.79%、35.77%（P<0.01)，MDA含量分别极显著下降了23.08%和21.46%（P<0.01)。Pearson相关性分析表明小麦肽的体内抗疲劳与抗氧化作用高度相关。结论：小麦肽具有显著的抗氧化和缓解疲劳作用，且抗氧化活性与抗疲劳作用高度相关。

Abstract: Objective: To investigate the in vitro antioxidant activity and in vivo anti-fatigue effect of wheat peptides. Methods: The oxidative stress model was established by treating L929 cells with H₂O₂. Then the cell survival rate, the activity of lactic dehydrogenase (LDH), superoxide dismutase (SOD) and glutathione peroxide dismutase (GSH-Px), and the content of malondialdehyde (MDA) were measured to evaluate the antioxidant activity of wheat peptides in vitro. In addition, the exhaustive swimming and freestyle swimming test was assessed. Then the exhaustive swimming time, the content of lactic acid (LA), blood urea nitrogen (BUN), muscle glycogen (MG) and MDA, the activity of SOD and GSH-Px were measured to study the anti-fatigue and antioxidant of wheat peptides in vivo. Results: The wheat peptides in the concentration range from 0.4 to 0.8 mg/mL could protect L929 cells against H₂O₂-induced oxidative damage by highly significantly increasing the survival rate（P<0.01). Compared to the model group, the activity of LDH of the wheat peptides at 0.6 and 0.8 mg/mL significantly decreased by 20.79% and 19.67%（P<0.05) , the activity of SOD was significantly （P<0.05) and highly significantly（P<0.01) increased by 83.21% and 95.19%, the activity of GSH-Px increased by 28.69% and 32.14%, and the content of MDA significantly decreased by 25.91% and 26.99%（P<0.05). Compared with the control group, the exhaustive swimming time of the wheat peptides at 2 and 8 mg/mL was significantly increased by 72.93% and 91.73%（P<0.01) , the content of LA significantly decreased by 24.65% and 25.16%（P<0.01) , the content of BUN highly significantly （P<0.01) and significantly（P<0.05) decreased by 19.74% and 17.78%, and the content of MG highly significantly increased by 48.63% and 56.85%（P<0.01) . At the same time, the activity of SOD and GSH-Px was highly significantly increased by 20.54% and 25.91%, 29.79% and 35.77%（P<0.01) , and the content of MDA was highly significantly decreased by 23.08% and 21.46%（P<0.01) . Pearson correlation analysis showed that the antifatigue effect of wheat peptides was highly correlated with its antioxidant activity. Conclusion: Wheat peptide had significant antioxidant activity and anti-fatigue effect, and the anti-fatigue effect of wheat peptide was correlated with antioxidant activity.

小麦肽的抗氧化与抗疲劳作用的研究

Study on the Antioxidant and Anti-fatigue Effect of Wheat Peptides