Abstract: A method for the determination of 37 veterinary drugs in animal-derived foods with QuEChERS by UPLC-MS /MS was established. The sample was extracted by 1% formic acid-acetonitrile precipitated protein, purified by PSA, C_18, NH₂ adsorbent. The separation was performed on Waters ACQUITY UPLC BEH C₁₈ (1.7 μm, 2.1 mm×100 mm) column. The mobile phase performed on 0.1% formic acid aqueous solution-methanol by gradient elution, electrospray ionization source (ESI) was applied and operated in the positive mode. Multiple reaction monitoring (MRM) mode was used to monitor ions. Matrix matching standard curve quantification by external standard method. Results showed that, an excellent linear relationship in the range of 1.0~200 μg/L was observed for all the analytes with a correlation coefficient larger than 0.9980. The recoveries at three concentrations ranged from 70.1% to 97.7%, with the RSD from 0.9% to 5.6%. The limits of quantification of the method were 0.01~0.54 μg/kg. This method was used to detect 152 batches of animal-derived food samples which purchased at the farmer’s market. Sulfadiazine and trimethoprim were detected in 3 batches of Oreochromis mossambicus, the remaining samples were not detected. The method was simple, rapid and had good precision, accuracy and quantitative limit. It would be suitable for the rapid determination of nitroimidazoles and their metabolites, sulfonamides in animal-derived foods.