Abstract: The aim of this study was to investigate the regulation of Potentilla fruticosa tea on blood lipid and gut micrebiota in hyperlipidemia rats(Wistar rats). The hyperlipidemia rat model was successfully bulit by inhacing with high-fat diet.Enzyme linked immunosorbent assay (ELISA) was used to investigate the effects of three kinds of Potentilla fruticosa tea on blood lipid in hyperlipidemia rats.Meanwhile, the diversity and structural changes of intestinal flora were analyzed by 16rS high-throughput sequencing.The results showed that compared with the model group, the HDL content of Potentilla fruticosa tea group was increased.There was significant difference in Potentilla fruticosa black tea group (P<0.05).The contents of TG, TC in Potentilla fruticosa flower tea, green tea and black tea groups were significantly decreased (P<0.05, P<0.01), and the content of LDL was decreased, but the difference was significant only in flower tea (P<0.05). At the same time, the results of intestinal flora showed that at the Phylum level, the abundance of dominant flora Firmicutes was down-regulated, and the abundance of Bacteroidetes was up-regulated. At thegenus level, the abundance of Lactobacillus and bacteroides in the intestinal tract of rats was significantly increased, while the abundance of unidentified_Ruminococcaceae, unidentified_Lachnospiraceae decreased significantly.Conclusion Potentilla fruticosa tea had the effect of regulating blood lipid and intestinal flora in hyperlipidemia rats, which had a tendency to recover to normal control group. It is speculated that Potentilla fruticosa tea may play a role in reducing blood lipid by interfering with lipid metabolism pathway by intestinal flora.