Abstract: In recent years, mycotoxin contamination in food has become a hot and difficult issue in the field of food safety. Immunoassay has the advantages of short detection time, simple operation steps, low cost and environmental friendliness, which is suitable for food safety detection. The research of new labeling materials-based immunoassay techniques in rapid detection of mycotoxin in cereal products is reviewed in this paper, including enzyme linked immunosorbent assay, immunochromatography, electrochemical immunosensor, immunochips, cytometric bead array based on immunoassay and time resolved fluorescence immunoassay. The advantages and disadvantages of the above immunoassay are systematically analyzed, which provides references for the application and development of immunoassay techniques in the detection of mycotoxins, and also provides new ideas for ensuring safety of grain products.