藜麦皂苷与纳米银配合物的制备及其协同抗菌性的研究

荆金金; 张若愚; 杜雪; 杜立冬; 刘兆利; 林冰洁; 张丰香; 薛鹏

doi:10.13386/j.issn1002-0306.2020060176

藜麦皂苷与纳米银配合物的制备及其协同抗菌性的研究

1. 潍坊医学院公共卫生学院, 山东潍坊 261053;
2. 潍坊医学院临床学院, 山东潍坊 261053

基金项目:

山东省自然基金（ZR2019PH043）。

详细信息

作者简介:
荆金金(1993-),女,硕士研究生,研究方向:食品营养与安全,E-mail:18353687571@163.com。

通讯作者:
薛鹏(1989-),男,博士,讲师,研究方向:中药化学,抗食源性致病菌功能因子筛选,E-mail:jplxp26@126.com。

中图分类号: TS210.1
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- 文章访问数: 271
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出版历程
- 收稿日期: 2020-06-14
- 网络出版日期: 2021-03-15
- 刊出日期: 2021-03-14

Synthesis and Synergistic Antibacterial Activity of Complexes of Quinoa Saponins with Silver Nanoparticles

1. School of Public Health of Weifang Medical University, Weifang 261053, China;
2. Clinical College of Weifang Medical University, Weifang 261053, China

摘要

摘要: 以藜麦皂苷和硝酸银为原料，通过生物还原法制备藜麦皂苷与纳米银配合物，并研究配合物的抑菌活性。利用紫外可见分光光度计（UV-vis光谱仪）、透射电子显微镜（TEM）对藜麦皂苷与纳米银配合物进行表征分析；通过抑菌圈实验、最低抑菌浓度（MIC）实验、最小杀菌浓度（MBC）实验等研究藜麦皂苷与纳米银配合物的协同抗菌性。结果表明，藜麦皂苷与纳米银配合物为球形，粒子直径大部分集中在10 nm附近，单分散性好；在420 nm处出现特征吸收峰。藜麦皂苷与纳米银配合物对食源性微生物有较好的抑制作用和协同抑制作用，其中对表皮葡萄球菌、金黄色葡萄球菌的抑制效果最好，抑菌直径分别为（11.33±0.58）、（10.67±1.15） mm，对正常胃黏膜上皮细胞（GES）及胃癌细胞（SGC）毒性较低。藜麦皂苷作为生物还原剂使纳米银的溶解性与分散性增强，保留了纳米银本身的广谱抗菌特性，并相互协同使纳米银抗菌活性增强。
- 藜麦皂苷 /
- 纳米银 /
- 配合物 /
- 抑菌作用 /
- 协同作用
Abstract: Quinoa saponins and silver nitrateas raw materials, the complexes of quinoa saponins with nano-silver were prepared by biological reduction method, and the antibacterial activity of the complexes was studied. The complexes of quinoa saponins and silver nanoparticles were characterized and analyzed by ultraviolet-visible spectrophotometer(UV-vis spectrophotometer) and transmission electron microscope(TEM). The synergistic antibacterial activity of quinoa saponins and silver nanoparticles were studied by bacteriostatic circle experiment, minimal inhibitory concentration(MIC) experiment, minimum bactericidal concentration(MBC) experiment and so on. The results showed that the complex of quinoa saponins with silver nanoparticles was spherical, and the diameter of the particles was mostly concentrated around 10 nm, with good dispersity. Characteristic absorption peaks appear at 420 nm. The complex of quinoa saponins and nano-silver preform had good inhibitory effect and synergistic inhibitory effect on foodborne microorganisms, and had good inhibitory effect on Staphylococcus epidermidis and Staphylococcus aureus, the bacteriostatic diameter were(11.33±0.58) and(10.67±1.15) mm, respectively. The low toxicity to normal gastric mucosal epithelial cell(GES) and gastric cancer cell(SGC). As a biological reducing agent, quinoa saponins enhanced the solubility and dispersion of silver nanoparticles, retained the broad-spectrum antibacterial properties of silver nanoparticles, and cooperated with each other to enhance their antibacterial properties.
- quinoa saponin /
- nano silver /
- complex /
- antibacterial effect /
- synergistic effect

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