富平尖柿果皮色素的理化及功能特性

梁宗瑶; 施宝珠; 魏园园; 任维维; 李珉梦; 梁连友; 段旭昌

doi:10.13386/j.issn1002-0306.2020060065

富平尖柿果皮色素的理化及功能特性

1. 西北农林科技大学食品科学与工程学院, 陕西咸阳 712100;
2. 西北农林科技大学, 富平现代农业综合试验示范站, 陕西渭南 711799

基金项目:

渭南市科技局-西北农林科技大学校际合作项目（K3380219082）；西北农林科技大学试验示范站（基地）科技创新与成果转化项目（TGZX2020-29）。

详细信息

作者简介:
梁宗瑶(1997-),女,硕士研究生,研究方向:天然产物提取及功能性,E-mail:2894761781@qq.com。

通讯作者:
段旭昌(1965-),男,博士,副教授,研究方向:天然产物及食品加工新技术,E-mail:duanxc1965@163.com。

中图分类号: TS201
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出版历程
- 收稿日期: 2020-06-07
- 网络出版日期: 2021-03-15
- 刊出日期: 2021-03-14

Physicochemical Properties and Functionality of ‘Fuping-Jianshi’ Persimmon Peel Pigment

1. College of Food Science and Engineering, Northwest A&F University, Xianyang 712100, China;
2. Fuping Modern Agriculture Comprehensive Demonstration Station, Northwest A&F University, Weinan 711799, China

摘要

摘要: 为了探索富平尖柿果皮色素的功能性及应用特性，本文采用溶剂提取纯化技术、光谱分析及体外抗氧化、抑菌、抑癌试验，研究了柿子皮色素的提取纯化、成分组成、理化特性及体外清除自由基、抑菌、对HepG2及MNK-45细胞体外增殖的影响。结果表明：富平尖柿果皮色素的提取率高达2.18%，色素中含有10种以上成分，其中β-胡萝卜素占52.31%，叶黄素占8.73%，玉米黄质占4.02%；光照、高温、氧化剂、Cu²⁺可导致柿子皮色素褪色，酸、Ca²⁺、Zn²⁺、Al³⁺可使柿子皮色素产生沉淀，还原剂和防腐剂对柿子皮色素有保护作用；柿子皮色素可清除DPPH·、·OH、ABTS⁺·，可螯合Fe²⁺，与V_C、β-胡萝卜素、叶黄素相比，抗氧化性能强弱依次为V_C>β-胡萝卜素>柿皮色素>叶黄素，柿子皮色素抗脂质过氧化能力随浓度增加而增强；柿子皮色素对沙门氏菌、金黄色葡萄球菌、大肠杆菌均有抑制作用，对李斯特菌、阪崎乳杆菌无抑制作用；柿皮色素对HepG2及MNK-45癌细胞体外增殖有一定抑制效果。富平尖柿果皮色素具有良好抗氧化、抑菌、抗癌细胞体外增殖性能，可作为天然功能性色素使用。
- 富平尖柿 /
- 柿皮色素 /
- 成分组成 /
- 理化稳定性 /
- 功能特性
Abstract: In order to explore the functionality and application characteristics of ‘Fuping-Jianshi’ persimmon peel pigment, the persimmon peel pigment was extracted and purified by solvent extraction and purification technology. The composition, physicochemical properties, free radical scavenging capacity, antibacterial properties, inhibitory effect on HepG2 and MNK-45 cell proliferation in vitro of the pigment were investigated by spectral analysis, antioxidant, anti-bacterial and anti-cancer cell proliferation experiments in vitro. The results showed that the extraction rate of the persimmon peel pigment was as high as 2.18%, and there were at least 10 kinds of components in the pigment, in which β-carotene accounted for 52.31%, lutein 8.73% and zeaxanthin 4.02%. Light, high temperature, oxidant and Cu²⁺ could cause fading of the pigment, acid, Ca²⁺, Zn²⁺, Al³⁺ could make the pigment precipitate, reducing agent and preservative had protective effect on the pigment. The pigment could scavenge DPPH·,·OH, ABTS⁺·, and chelate Fe²⁺. Compared with V_C, β-carotene and lutein, the order of antioxidant activity was V_C>β-carotene>the pigment>lutein, the anti-lipid peroxidation ability of the pigment increased with the increasing of the pigment concentration. The pigment could inhibit the growth of Salmonella, Staphylococcus aureus and Escherichia coli, and had no inhibitory effect on Listeria and Lactobacillus sakazakii. The pigment could inhibit the proliferation of HepG2 and MNK-45 cancer cells in vitro. As a conclusion: ‘Fuping-Jianshi’ persimmon peel pigment had good antioxidative, antibacterial and anticancer activities in vitro, and can be used as natural functional pigment.
- Fuping-Jianshi' persimmon /
- persimmon peel pigment /
- composition /
- physicochemical stability /
- functional properties

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