Abstract: Deep eutectic solvent (DES) is a mixture formed by the combination of hydrogen-bond acceptor (HBA) and hydrogen-bond donor (HBD) with a certain stoichiometric ratio.The melting point of the whole system is lower than that of a single component due to the intermolecular hydrogen bond interaction. It is a non-toxic, inexpensive, easy preparation and biodegradable green solvent and can extract some chemical components from different food matrix.At present, the combination of DES and ultrasonic technology has become a new extraction tool in the food industry. This paper mainly summarizes the classification of DES, preparation method of DES and the influence of the ultrasound-assisted extraction with deep eutectic solvent. At the same time, its application in extraction of active ingredients and pretreatment of food analysis is also discussed.