Effect of srfAC Gene Knockout on the Antibacterial Activity of Bacillus amyloliquefaciens
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摘要: 本实验为了探究表面活性素（Surfactin）对解淀粉芽胞杆菌（Bacillus amyloliquefaciens）抑制金黄色葡萄球菌（Staphylococcus aureus）的影响，在B.amyloliquefaciens HZ-12中敲除了Surfactin合成关键基因srfAC。结果显示，缺失菌B.amyloliquefaciens HZ-12ΔsrfAC对金黄色葡萄球菌的抑制效果明显提高，相对抑制率比对照菌提高了64%。对工程菌B.amyloliquefaciens HZ-12ΔsrfAC的培养基碳氮源进行了优化，在玉米淀粉30 g/L和豆粕40 g/L的条件下，B.amyloliquefaciens HZ-12ΔsrfAC的生物量达到3.3×1010 CFU/mL，是优化前的2.23倍。本研究首次证实敲除srfAC基因可明显提高B.amyloliquefaciens对S.aureus的抑制作用，并获得了高效培养B.amyloliquefaciens HZ-12ΔsrfAC的培养基配方，具有重要的理论意义和潜在的应用价值。Abstract: To investigate the effect of surfactin on the inhibition activity of Bacillus amyloliquefaciens against Staphylococcus aureus,the key gene srfAC for surfactin synthesis was knocked out from B. amyloliquefaciens HZ-12. The results showed that the inhibitory activity of the mutant B. amyloliquefaciens HZ-12ΔsrfAC against S. aureus was significantly improved,and the relative inhibition rate was increased by 64%. Furthermore,the medium carbon and nitrogen sources of engineering strain B. amyloliquefaciens HZ-12ΔsrfAC were optimized. Under 30 g/L of corn starch and 40 g/L of soybean meal,the biomass of B. amyloliquefaciens HZ-12ΔsrfAC reached 3.3×1010 CFU/mL,2.23 times than that without optimization. This study confirmed that srfAC gene knockout could significantly improve the inhibitory activity of B. amyloliquefaciens against S. aureus for the first time,and the medium for efficient cell growth was obtained. The results had improtant theoretical significance and potential application value.
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