Study on the Antioxidant Capacity of Extracellular Polysaccharides of Pleurotus djamor in vitro and in Hyperlipidemia Mice
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摘要: 目的：探究红平菇胞外多糖（Extracellular polysaccharides，EPS）体外抗氧化能力及抵抗高血脂小鼠体内肝脏组织氧化损伤的功效。方法：通过EPS对O2-·、DPPH自由基和·OH的清除力，测定其体外抗氧化能力；构建高血脂小鼠模型，分为空白对照组（NC）、高血脂模型组（MC）、阳性对照组（PC）、EPS高、低剂量组；NC组随机分配10只正常小鼠，剩余组每组随机分配高血脂模型小鼠10只，干预14 d后，测定EPS对高血脂小鼠肝脏组织内谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）、过氧化脂质（LPO）活性的影响，衡量其体内抗氧化能力。同时，观察小鼠的肝脏部位病理学切片，探究EPS对受到氧化应激损伤的肝脏细胞的修复能力。结果：EPS对O2-·、DPPH自由基和·OH的饱和清除率分别达到55.05%、64.09%和28.91%。EPS可以显著提高（P<0.05）高血脂小鼠肝脏组织抗氧化酶系含量，显著降低（P<0.05）脂质过氧化物活性，同时对肝脏氧化应激损伤具有较好的修复作用。结论：EPS具有较强的体外抗氧化作用，并对高血脂小鼠肝脏组织表现出较好的抗氧化能力及氧化损伤修复能力。Abstract: Objective:To investigate the antioxidant capacity of extracellular polysaccharides(EPS)of Pleurotus djamor in vitro and its efficacy in resisting oxidative damage of liver tissue in hyperlipidemia mice.Methods:The scavenging activity of EPS on O2-·,DPPH radical and ·OH was determined,and its antioxidant activity in vitro was determined.The hyperlipidemia mice model was established by gavage of high-fat feed:Blank control group(NC),hyperlipidemia model group(MC),positive control group(PC),high and low dose EPS group. 10 normal mice were randomly assigned to the NC group and 10 hyperlipidemia model mice were randomly assigned to the remaining group after 14 days of intervention.The effects of EPS on the activities of glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),catalase(CAT),malondialdehyde(MDA)and lipid peroxide(LPO)in liver tissue of hyperlipidemia mice were measured to measure their antioxidant capacity in vivo. At the same time,the pathological sections of the liver of mice were observed to explore the repair ability of EPS to liver cells damaged by oxidative stress. Results:The saturation scavenging rates of EPS to O2-·,DPPH radical and·OH reached 55.05%,64.09% and 28.91%,respectively. EPS could significantly increase(P<0.05)the content of antioxidant enzymes in liver tissue of hyperlipemia mice,significantly reduce(P<0.05)lipid peroxide activity,and had good repair effect on liver oxidative stress injury.Conclusion:Exopolysaccharides of Pleurotus djamor has strong antioxidant effect in vitro,and has better antioxidant ability and oxidative damage repair ability on liver tissue of hyperlipidemia mice.
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[1] 周紫燕,王坦,洪晓鹏.抗氧化天然活性多糖[J].日用化学品学,2012,35:24-26,34. [2] HuYuNing,SungTzuJung,ChouChunHsu,et al. Characterization and antioxidant activities of yellow strain flammulina velutipes(Jinhua Mushroom)polysaccharides and their effects on ROS content in L929 Cell[J].Antioxidants,2019,8(8):298-298.
[3] 张聪,詹雪,赵丹丹.食用真菌多糖抗肿瘤活性研究进展[J].科学技术创新,2019(35):14-15. [4] Duoduo Xu,Haiyang Wang,Wei Zheng,et al. Characterization and immunomodulatory activities of polysaccharide isolated from Pleurotus eryngii[J]. International Journal of Biological Macromolecules,2016,92.
[5] 刘红波,钱益平,肖宁. 食用菌提取物的化学成分及其复方药效学研究[J]. 中国食用菌,2019(4):135-138. [6] 罗磊,张冰洁,韦倩倩,等. 金银花黄酮对过氧化氢诱导RAW264.7巨噬细胞损伤的保护作用[J]. 中国食品学报,2019,19(5):18-25. [7] Thomas F Lüscher,Ulf Landmesser,Arnold von Eckardstein,et al. High-density lipoprotein:Vascular protective effects,dysfunction,and potential as therapeutic target[J]. Circulation Research,2014,114(1):171-182.
[8] 李重邑,陈琳方,刘国文.高浓度非酯化脂肪酸诱导Ⅱ型酮病奶牛氧化应激[J/OL].吉林农业大学学报 [9] Stojic Isidora M,Zivkovic Vladimir I,Srejovic Ivan M,et al. Cisplatin and cisplatin analogues perfusion through isolated rat heart:The effects of acute application on oxidative stress biomarkers[J]. Molecular and Cellular Biochemistry,2018,439(1-2).
[10] lhami Gülçin. Antioxidant activity of l-adrenaline:A structure-activity insight[J]. Chemico-Biological Interactions,2008,179(2).
[11] 高静.天然抗氧化剂及其协同作用[J].食品安全质量检测学报,2020(11):1859-1864. [12] Q Wang,H Li,TT Chen,et al. Yield,polysaccharides content and antioxidant properties of Pleurotus abalonus and Pleurotus geesteranus produced on asparagus straw as substrate[J]. Scientia Horticulturae,2012,134(1):222-226.
[13] 杨岚,邱树毅,卢卫红.真菌多糖的抗氧化活性研究[J]. 贵州师范大学学报(自然科学版),2017,35:95-99,119. [14] Yan Jingmin,Zhu Lei,Qu Yunhe,et al. Analyses of active antioxidant polysaccharides from four edible mushrooms[J]. International Journal of Biological Macromolecules,2019,123.
[15] 李艳芳,王相刚,张立伟.观赏食药用菌制作的初步研究[J].陕西农业科学,2020,66:80-82. [16] 张建军. 红平菇菌丝体多糖的提取、结构分析和抗糖尿病作用研究[D]. 泰安:山东农业大学,2016. [17] 孙学茹. 蒙古口蘑胞外多糖提取、纯化及生物活性研究[D].呼和浩特:内蒙古大学,2019. [18] 张建军. 姬菇SS-01胞外多糖及胞内锌多糖的提取和抗氧化活性[D].泰安:山东农业大学,2012. [19] 陈静,陈乃东,郑诠.四种石斛的根、茎、叶主要成分及其DPPH自由基清除能力对比[J].北方园艺,2020(10):128-133. [20] Zhou S H,Fang Z X,Lü Y,et al. Phenolics and antioxidant properties of bayberry(Myrica rubra Sieb. et Zucc.)pomace[J]. Food Chemistry,2009,112(2):394-399.
[21] Smirnoff Nicholas,Cumbes Quinton J. Hydroxyl radical scavenging activity of compatible solutes[J]. Pergamon,1989,28(4):1057-1060.
[22] 徐望龙,李云贵,孙林军,等.山银花黄酮粗提物抗氧化活性的体外观察[J].中成药,2014,36:1292-1294. [23] 蔡丹燕,祁龙凯,林励.佛手叶总黄酮超声提取工艺优化及其抗氧化活性研究[J].广州中医药大学学报,2015,32:308-312,341 ,385.
[24] Samir Zeroual,Ismail Daoud,Randa Gaouaoui,et al. In vitro and molecular docking studies of DPPH with Phoenix dactylifera L.(Deglet-Nour)crude fruits extracts and evaluation of their antioxidant activity[J]. Asian J Research Chem,2020,13(1):52-59.
[25] 韦献雅,殷丽琴,钟成,等.DPPH法评价抗氧化活性研究进展[J].食品科学,2014,35:317-322. [26] 吴春,陈林林.菟丝子黄酮体外清除自由基活性的研究[J].天然产物研究与开发,2005,17(5):553-556. [27] Jong-Min Lee,Hoonjeong Kwon,Hoon Jeong,et al. Inhibition of lipid peroxidation and oxidative DNA damage by Ganoderma lucidum[J]. Phytotherapy Research,2001,15(3):245-249.
[28] 顾盼盼. 锌、NAC对镉中毒鸡肝脏、肾脏病理损伤的影响[D].太原:山西农业大学,2018. [29] 宋娜,苏东峰.麦冬多糖对糖尿病围绝经期大鼠血清SOD、GSH-Px、CAT、MDA水平的影响[J].东南大学学报(医学版),2019,38:979-984. [30] Jingsheng He,Bo Huang,Xiaoquan Ban,et al. In vitro and in vivo antioxidant activity of the ethanolic extract from Meconopsis quintuplinervia[J]. Journal of Ethnopharmacology,2012,141(1):104-110.
[31] Zhen Bao,Weijun Chen,Fan Pan,et al. Role of mitofusin 2 in the protective effect of breviscapine againsthepatic ischemia/reperfusion injury in rats[J]. Experimental and Therapeutic Medicine,2018,15(4):3582-3588.
[32] Zhao Ma,Fangyuan Cui,Xia Gao,et al. Purification,characterization,antioxidant activity and anti-aging of exopolysaccharides by Flammulina velutipes SF-06[J]. Antonie van Leeuwenhoek,2015,107(1):73-82.
[33] Jianjun Zhang,Min Liu,Yongheng Yang,et al. Purification,characterization and hepatoprotective activities of mycelia zinc polysaccharides by Pleurotus djamor[J]. Carbohydrate Polymers,2016,136(6):588-597.
[34] Shangshang Li,Hui Liu,Wenshuai Wang,et al. Antioxidant and anti-aging effects of acidic-extractable polysaccharides by Agaricus bisporus[J]. International Journal of Biological Macromolecules,2018,106.
[35] Xue C,Fang Y,Lin H,et al. Chemical characters and antioxidative properties of sulfated polysaccharides from laminaria japonica[J].J Appl Phycol,2001,13(1):67-70.
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